| RRC ID |
38258
|
| Author |
Nozaki H, Yanagida M, Koide K, Shiotani K, Kinoshita M, Kobayashi Y, Watarai S, Nakamura K, Suzuki A, Ariga T, Kushi Y.
|
| Title |
Production and characterization of monoclonal antibodies specific to lactotriaosylceramide.
|
| Journal |
Glycobiology
|
| Abstract |
We have established hybridoma cell lines producing monoclonal antibodies (mAbs) directed to N-acetylglucosaminylβ1-3galactose (GlcNAcβ1-3Gal) residue by immunizing BALB/c mice with lactotriaosylceramide (Lc(3)Cer). These obtained hybridoma cells, specific to Lc(3)Cer, were dual immunoglobulin (Ig)-producing cells which secreted both IgM and IgG molecules as antibodies. The established mAbs are able to react with not only Lc(3)Cer but also GlcNAcβ1-3-terminal glycosphingolipids (GSLs) despite branching or lactosamine chain lengths and human transferrin with terminal GlcNAc residues. Comparison of the variable regions of the cloned IgM and IgG by reversed transcription-polymerase chain reaction analysis confirmed that the variable regions determine the specificity, the other amino acids are conserved, and these mAbs are encoded by J558 and Vκ-21family genes. Furthermore, we have analyzed the expression of GSLs with GlcNAcβ1-3 epitope in acute leukemia cell lines and mouse fetal tissues using these mAbs, in which antigens were distributed comparatively. These mAbs are useful for studying the precise distribution of GlcNAcβ1-3Gal-terminating GSL expression in tissues as well as for detecting GSLs carrying terminal GlcNAcβ1-3Gal carbohydrate structure.
|
| Volume |
20(12)
|
| Pages |
1631-42
|
| Published |
2010-12-1
|
| DOI |
10.1093/glycob/cwq117
|
| PII |
cwq117
|
| PMID |
20693232
|
| MeSH |
Animals
Antibodies, Monoclonal, Murine-Derived / biosynthesis
Antibodies, Monoclonal, Murine-Derived / genetics
Antibodies, Monoclonal, Murine-Derived / immunology*
Antibody Specificity / genetics
Antibody Specificity / immunology*
Female
HL-60 Cells
Humans
Immunoglobulin G / biosynthesis
Immunoglobulin G / genetics
Immunoglobulin G / immunology*
Immunoglobulin M / biosynthesis
Immunoglobulin M / genetics
Immunoglobulin M / immunology*
Immunoglobulin Variable Region / biosynthesis
Immunoglobulin Variable Region / genetics
Immunoglobulin Variable Region / immunology*
K562 Cells
Lactosylceramides / biosynthesis
Lactosylceramides / genetics
Lactosylceramides / immunology*
Mice
Mice, Inbred BALB C
U937 Cells
|
| IF |
4.06
|
| Times Cited |
7
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HL60(RCB0041)
K562(RCB0027)
U-937 DE-4(RCB0435) |
