RRC ID 38265
著者 Cho Y, Honda S, Yoshizawa Y, Takagaki K, Usui K, Shibuya A.
タイトル Requirement of the cytoplasmic portion for dimer formation of Fcalpha/micro receptor expressed on cell surface.
ジャーナル Mol Immunol
Abstract Fcalpha/mu receptor (Fcalpha/muR), an Fc receptor for IgA and IgM, is the only Fc receptor for IgM identified on hematopoietic cells in human and rodents and for IgA in rodents. Fcalpha/microR is a type 1 transmembrane protein containing one immunoglobulin-like domain in the extracellular portion. Both human and mouse Fcalpha/microR mediate endocytosis of the ligands IgA and IgM, for which the cytoplasmic portion of Fcalpha/microR is responsible. However, molecular characteristics of Fcalpha/muR involved in the function have been incompletely understood. Here, we show that both monomeric and dimeric Fcalpha/microR are expressed in a mouse B cell line BCL1-B20 and BW5147 or Ba/F3 transfectants stably expressing Fcalpha/microR. We also show that the dimeric, but not monomeric, Fcalpha/microR is preferentially localized to the cell surface of the transfectants. BW5147 transfectant expressing mutant Fcalpha/microR lacking the cytoplasmic portion expressed only the monomeric Fcalpha/microR. These results suggest that the cytoplasmic portion is required for the dimer formation and thus for efficient cell surface expression of Fcalpha/microR.
巻・号 47(4)
ページ 878-82
公開日 2010-1-1
DOI 10.1016/j.molimm.2009.10.016
PII S0161-5890(09)00788-3
PMID 19945166
MeSH Animals Cell Membrane / metabolism* Cytoplasm / metabolism* Humans Ligands Mice Mutant Proteins / chemistry Mutant Proteins / metabolism Protein Multimerization* Receptors, Fc / chemistry* Receptors, Fc / metabolism* Structure-Activity Relationship Transfection
IF 3.641
引用数 6
WOS 分野 IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞