| RRC ID |
38273
|
| Author |
Takada K, Saito M, Tsuzukibashi O, Kawashima Y, Ishida S, Hirasawa M.
|
| Title |
Characterization of a new serotype g isolate of Aggregatibacter actinomycetemcomitans.
|
| Journal |
Mol Oral Microbiol
|
| Abstract |
Aggregatibacter actinomycetemcomitans is usually isolated from the oral cavity where it is associated with active periodontitis. The species can be divided into six serotypes (a-f) according to their surface carbohydrate antigens. However, some clinical isolates cannot be grouped within these six serotypes. Gram-negative, facultative anaerobic, catalase-positive coccobacilli were isolated from a patient with periodontitis and identified by employing genetic, biochemical and serological analyses. Phenotypic data identified the isolate as A. actinomycetemcomitans. Serotype-specific polysaccharide antigen from the isolate was untypeable by immunodiffusion testing in comparison with reference A. actinomycetemcomitans serotype a to f strains. Biofilm formation by the isolate was strong but cytotoxic activity was low. Gas chromatography/mass spectroscopy analysis of partially methylated alditol acetates from surface polysaccharide showed the presence of 2,4-di-O-methyl-rhamnose and 2,3,6-tri-O-methyl-glucose, with a 1 : 1 m ratio. The (1)H- and (13)C-nuclear magnetic resonance spectra of the antigen showed that both constituent glycoses had alpha-anomeric configuration. It is proposed that the untyped strain is a new A. actinomycetemcomitans serotype, designated serotype g.
|
| Volume |
25(3)
|
| Pages |
200-6
|
| Published |
2010-6-1
|
| DOI |
10.1111/j.2041-1014.2010.00572.x
|
| PII |
OMI572
|
| PMID |
20536747
|
| MeSH |
Aggregatibacter actinomycetemcomitans / classification*
Biofilms
Cell Survival
Chromatography, Gas
Chronic Periodontitis / microbiology*
DNA, Bacterial / analysis
HL-60 Cells / microbiology
Humans
Mass Spectrometry
Periodontal Pocket / microbiology
Polymerase Chain Reaction
Polysaccharides, Bacterial / chemistry*
Serotyping / methods
Sugar Alcohols / analysis
|
| IF |
2.905
|
| Times Cited |
28
|
|
WOS Category
|
DENTISTRY, ORAL SURGERY & MEDICINE
MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
HL60(RCB0041) |
