| RRC ID |
38279
|
| 著者 |
Horie M, Nishio K, Kato H, Shinohara N, Nakamura A, Fujita K, Kinugasa S, Endoh S, Yamamoto K, Yamamoto O, Niki E, Yoshida Y, Iwahashi H.
|
| タイトル |
In vitro evaluation of cellular responses induced by stable fullerene C60 medium dispersion.
|
| ジャーナル |
J Biochem
|
| Abstract |
Because of the expansion of the functionalities available for modification of fullerene C60 and its derivatives, their uses are increasing. However, the consequences of fullerene exposure to human health have not been fully studied. In vitro experiments are useful for risk assessment and for understanding potential applications. However, the insolubility of pristine C60 in water makes the in vitro evaluation of cellular responses difficult. To overcome this problem, we prepared a stable and uniform C60-medium dispersion for in vitro examinations. In addition, we examined the effect of the C60-medium dispersion on human keratinocyte HaCaT cells and human lung carcinoma A549 cells to understand the cellular responses induced by exposure to C60. Results indicated that exposure to C60 did not affect cell viability; neither apoptosis nor necrosis were induced, while cell proliferation was inhibited. Furthermore, intracellular oxidative stress was induced by C60 exposure in both HaCaT and A549 cells. Transmission electron microscopy indicated the cellular uptake of C60 aggregates. The results obtained from this study indicate that C60 has oxidative stress induction potential. Further examinations including in vivo studies are necessary for a more accurate evaluation of biological influences by C60.
|
| 巻・号 |
148(3)
|
| ページ |
289-98
|
| 公開日 |
2010-9-1
|
| DOI |
10.1093/jb/mvq068
|
| PII |
mvq068
|
| PMID |
20576621
|
| MeSH |
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Drug Stability
Fullerenes / pharmacokinetics
Fullerenes / pharmacology*
Humans
Keratinocytes / cytology
Keratinocytes / drug effects
Oxidative Stress / drug effects
Risk Assessment
|
| IF |
2.476
|
| 引用数 |
37
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
