論文 - 詳細
| RRC ID | 38285 |
|---|---|
| 著者 | El-Beialy W, Galal N, Deyama Y, Yoshimura Y, Suzuki K, Tei K, Totsuka Y. |
| タイトル | Regulation of human and pig renal Na(+),K (+)-ATPase activity by tyrosine phosphorylation of their alpha(1)-subunits. |
| ジャーナル | J Membr Biol |
| Abstract |
Modulation of the physiologically influential Na(+),K(+)-ATPase is a complex process involving a wide variety of factors. To determine the possible effects of the protein tyrosine phosphatase (PTP) inhibitors dephostatin and Et-3,4-dephostatin on human and pig, renal cells and enzymatic extracts, we treated our samples (15 min-24 h) with those PTP inhibitors (0-100 microM). PTP inhibitors were found to possess a concentration-dependent inhibition of Na(+),K(+)-ATPase activity in both human and pig samples. The inhibition was similarly demonstrated on all cellular, microsomal fraction and purified Na(+),K(+)-ATPase levels. Despite rigorous activity recovery attempts, the PTP inhibitors' effects were sustained on Na(+),K(+)-ATPase activity. Western blotting experiments revealed the expression of both alpha(1)- and beta(1)-subunits in both human and pig tissues. alpha(1)-Subunits possessed higher tyrosine phosphorylation levels with higher concentrations of PTP inhibitors. Meanwhile, serine/threonine residues of both alpha(1)- and beta(1)-subunits demonstrated diminished phosphorylation levels upon dephostatin treatment. Accordingly, we provide evidence that Na(+),K(+)-ATPase can be regulated through tyrosine phosphorylation of primarily their alpha(1)-subunits, using PTP inhibitors. |
| 巻・号 | 233(1-3) |
| ページ | 119-26 |
| 公開日 | 2010-2-1 |
| DOI | 10.1007/s00232-010-9231-z |
| PMID | 20130847 |
| MeSH | Animals Blotting, Western Catecholamines / pharmacology Cell Line, Tumor Flow Cytometry Humans Hydroquinones / pharmacology Kidney / enzymology* Nitroso Compounds / pharmacology Phosphorylation / drug effects Protein Subunits / metabolism* Protein Tyrosine Phosphatases / antagonists & inhibitors Sodium-Potassium-Exchanging ATPase / metabolism* Swine Tyrosine / metabolism* |
| IF | 1.877 |
| 引用数 | 7 |
| WOS 分野 | PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | OS-RC-2(RCB0735) |