RRC ID 38288
著者 Shishido T, Mieda H, Hwang SY, Nishimura Y, Tanaka T, Ogino C, Fukuda H, Kondo A.
タイトル Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells.
ジャーナル Bioorg Med Chem Lett
Abstract A novel HER2-targeted carrier was developed using bionanocapsules (BNCs). Bionanocapsules (BNCs) are 100-nm hollow nanoparticles composed of the L-protein of hepatitis B virus surface antigen. An affibody of HER2 was genetically displayed on the BNC surface (Z(HER2)-BNC). For the investigation of binding affinity, Z(HER2)-BNC was incubated with the cancer cell lines SK-BR-3 (HER2 positive), and MDA-MB-231 (HER2 negative). For analysis of HER2 targeting specificity, Z(HER2)-BNC or Z(WT)-BNC (without affibody) was incubated with both SK-BR-3 and MDA-MB-231 cells by time lapse and concentration. For the delivery of encapsulated molecules (calcein), fluorescence of Z(HER2)-BNC mixed with liposomes was also compared with that of Z(WT)-BNC and nude liposomes by incubation with SK-BR-3 cells. As a result, Z(HER2)-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment.
巻・号 20(19)
ページ 5726-31
公開日 2010-10-1
DOI 10.1016/j.bmcl.2010.08.011
PII S0960-894X(10)01121-2
PMID 20801029
MeSH Breast Neoplasms / drug therapy Cell Line, Tumor Drug Carriers / chemistry Female Fluoresceins / chemistry Hepatitis B Antigens / genetics Hepatitis B Antigens / metabolism Humans Nanoparticles / administration & dosage* Receptor, ErbB-2 / metabolism* Recombinant Fusion Proteins / administration & dosage* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 2.572
引用数 20
WOS 分野 CHEMISTRY, MEDICINAL CHEMISTRY, ORGANIC
リソース情報
ヒト・動物細胞