論文 - 詳細
| RRC ID | 38288 |
|---|---|
| 著者 | Shishido T, Mieda H, Hwang SY, Nishimura Y, Tanaka T, Ogino C, Fukuda H, Kondo A. |
| タイトル | Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells. |
| ジャーナル | Bioorg Med Chem Lett |
| Abstract |
A novel HER2-targeted carrier was developed using bionanocapsules (BNCs). Bionanocapsules (BNCs) are 100-nm hollow nanoparticles composed of the L-protein of hepatitis B virus surface antigen. An affibody of HER2 was genetically displayed on the BNC surface (Z(HER2)-BNC). For the investigation of binding affinity, Z(HER2)-BNC was incubated with the cancer cell lines SK-BR-3 (HER2 positive), and MDA-MB-231 (HER2 negative). For analysis of HER2 targeting specificity, Z(HER2)-BNC or Z(WT)-BNC (without affibody) was incubated with both SK-BR-3 and MDA-MB-231 cells by time lapse and concentration. For the delivery of encapsulated molecules (calcein), fluorescence of Z(HER2)-BNC mixed with liposomes was also compared with that of Z(WT)-BNC and nude liposomes by incubation with SK-BR-3 cells. As a result, Z(HER2)-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment. |
| 巻・号 | 20(19) |
| ページ | 5726-31 |
| 公開日 | 2010-10-1 |
| DOI | 10.1016/j.bmcl.2010.08.011 |
| PII | S0960-894X(10)01121-2 |
| PMID | 20801029 |
| MeSH | Breast Neoplasms / drug therapy Cell Line, Tumor Drug Carriers / chemistry Female Fluoresceins / chemistry Hepatitis B Antigens / genetics Hepatitis B Antigens / metabolism Humans Nanoparticles / administration & dosage* Receptor, ErbB-2 / metabolism* Recombinant Fusion Proteins / administration & dosage* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism |
| IF | 2.572 |
| 引用数 | 20 |
| WOS 分野 | CHEMISTRY, MEDICINAL CHEMISTRY, ORGANIC |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SK-BR-3(RCB2132) |