| RRC ID |
38288
|
| 著者 |
Shishido T, Mieda H, Hwang SY, Nishimura Y, Tanaka T, Ogino C, Fukuda H, Kondo A.
|
| タイトル |
Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells.
|
| ジャーナル |
Bioorg Med Chem Lett
|
| Abstract |
A novel HER2-targeted carrier was developed using bionanocapsules (BNCs). Bionanocapsules (BNCs) are 100-nm hollow nanoparticles composed of the L-protein of hepatitis B virus surface antigen. An affibody of HER2 was genetically displayed on the BNC surface (Z(HER2)-BNC). For the investigation of binding affinity, Z(HER2)-BNC was incubated with the cancer cell lines SK-BR-3 (HER2 positive), and MDA-MB-231 (HER2 negative). For analysis of HER2 targeting specificity, Z(HER2)-BNC or Z(WT)-BNC (without affibody) was incubated with both SK-BR-3 and MDA-MB-231 cells by time lapse and concentration. For the delivery of encapsulated molecules (calcein), fluorescence of Z(HER2)-BNC mixed with liposomes was also compared with that of Z(WT)-BNC and nude liposomes by incubation with SK-BR-3 cells. As a result, Z(HER2)-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment.
|
| 巻・号 |
20(19)
|
| ページ |
5726-31
|
| 公開日 |
2010-10-1
|
| DOI |
10.1016/j.bmcl.2010.08.011
|
| PII |
S0960-894X(10)01121-2
|
| PMID |
20801029
|
| MeSH |
Breast Neoplasms / drug therapy
Cell Line, Tumor
Drug Carriers / chemistry
Female
Fluoresceins / chemistry
Hepatitis B Antigens / genetics
Hepatitis B Antigens / metabolism
Humans
Nanoparticles / administration & dosage*
Receptor, ErbB-2 / metabolism*
Recombinant Fusion Proteins / administration & dosage*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
|
| IF |
2.572
|
| 引用数 |
20
|
|
WOS 分野
|
CHEMISTRY, MEDICINAL
CHEMISTRY, ORGANIC
|
| リソース情報 |
| ヒト・動物細胞 |
SK-BR-3(RCB2132) |
