Reference - Detail
| RRC ID | 38291 |
|---|---|
| Author | Akutsu N, Yamamoto H, Sasaki S, Taniguchi H, Arimura Y, Imai K, Shinomura Y. |
| Title | Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma. |
| Journal | World J Gastroenterol |
| Abstract |
AIM:To clarify the association of glypican-3 (GPC3) expression with Wnt and other growth signaling molecules in hepatocellular carcinoma (HCC). METHODS:Expression of GPC3, Wnt, matrix metalloproteinases (MMPs), sulfatase (SULF)1, SULF2, and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcription-polymerase chain reaction, immunoblotting, and/or immunostaining. Expression of various genes in GPC3 siRNA-transfected HCC cells was analyzed. RESULTS:GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels were significantly higher in patients with HCC than in non-HCC subjects (P < 0.05). Altered expressions of various MMPs and growth signaling molecules, some of which were correlated with GPC3 expression, were observed in HCCs. Down-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2, MMP14, fibroblast growth factor receptor 1, insulin-like growth factor 1 receptor. GPC3 expression was significantly correlated with nuclear/cytoplasmic localization of beta-catenin. CONCLUSION:These results suggest that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC. |
| Volume | 16(28) |
| Pages | 3521-8 |
| Published | 2010-7-28 |
| DOI | 10.3748/wjg.v16.i28.3521 |
| PMID | 20653060 |
| PMC | PMC2909551 |
| MeSH | Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor Glypicans / metabolism* Humans Intercellular Signaling Peptides and Proteins / metabolism* Liver Neoplasms / metabolism* Matrix Metalloproteinases / metabolism Sulfatases Sulfotransferases / metabolism Wnt Proteins / metabolism |
| IF | 3.665 |
| Times Cited | 23 |
| WOS Category | GASTROENTEROLOGY & HEPATOLOGY |
| Resource | |
| Human and Animal Cells | Li-7(RCB1941) |