RRC ID 38292
著者 Matsui K, Maruo Y, Sato H, Takeuchi Y.
タイトル Combined effect of regulatory polymorphisms on transcription of UGT1A1 as a cause of Gilbert syndrome.
ジャーナル BMC Gastroenterol
Abstract BACKGROUND:Gilbert syndrome is caused by defects in bilirubin UDP-glucuronosyltransferase (UGT1A1). The most common variation believed to be involved is A(TA)7TAA. Although several polymorphisms have been found to link with A(TA)7TAA, the combined effect of regulatory polymorphisms in the development of Gilbert syndrome remains unclear.
METHODS:In an analysis of 15 patients and 60 normal subjects, we detected 14 polymorphisms and nine haplotypes in the regulatory region. We classified the 4-kbp regulatory region of the patients into: the TATA box including A(TA)7TAA; a phenobarbital responsive enhancer module including c.-3275T>G; and a region including other ten linked polymorphisms. The effect on transcription of these polymorphisms was studied.
RESULTS:All haplotypes with A(TA)7TAA had c.-3275T>G and additional polymorphisms. In an in-vitro expression study of the 4-kbp regulatory region, A(TA)7TAA alone did not significantly reduce transcription. In contrast, c.-3275T>G reduced transcription to 69% of that of wild type, and the linked polymorphisms reduced transcription to 88% of wild type. Transcription of the typical regulatory region of the patients was 56% of wild type. Co-expression of constitutive androstane receptor (CAR) increased the transcription of wild type by a factor of 4.3. Each polymorphism by itself did not reduce transcription to the level of the patients, however, even in the presence of CAR.
CONCLUSIONS:These results imply that co-operation of A(TA)7TAA, c.-3275T>G and the linked polymorphisms is necessary in causing Gilbert syndrome.
巻・号 10
ページ 57
公開日 2010-6-8
DOI 10.1186/1471-230X-10-57
PII 1471-230X-10-57
PMID 20529348
PMC PMC2894006
MeSH Asian People / genetics Case-Control Studies Constitutive Androstane Receptor Gilbert Disease / genetics* Gilbert Disease / metabolism Glucuronosyltransferase / genetics* Haplotypes / genetics Humans Japan Polymorphism, Genetic / genetics* Receptors, Cytoplasmic and Nuclear / metabolism Transcription, Genetic* White People / genetics
IF 2.489
引用数 20
WOS 分野 GASTROENTEROLOGY & HEPATOLOGY
リソース情報
ヒト・動物細胞