| RRC ID |
38299
|
| 著者 |
Sudo K, Yasuda J, Nakamura Y.
|
| タイトル |
Gene expression profiles of cryopreserved CD34(+) human umbilical cord blood cells are related to their bone marrow reconstitution abilities in mouse xenografts.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Human umbilical cord blood (UCB) cells are an alternative source of hematopoietic stem cells for treatment of leukemia and other diseases. It is very difficult to assess the quality of UCB cells in the clinical situation. Here, we sought to assess the quality of UCB cells by transplantation to immunodeficient mice. Cryopreserved CD34(+) UCB cells from twelve different human donors were transplanted into sublethally irradiated NOD/shi-scid Jic mice. In parallel, the gene expression profiles of the UCB cells were determined from oligonucleotide microarrays. UCB cells from three donors failed to establish an engraftment in the host mice, while the other nine succeeded to various extents. Gene expression profiling indicated that 71 genes, including HOXB4, C/EBP-beta, and ETS2, were specifically overexpressed and 23 genes were suppressed more than 2-fold in the successful UCB cells compared to those that failed. Functional annotation revealed that cell growth and cell cycle regulators were more abundant in the successful UCB cells. Our results suggest that hematopoietic ability may vary among cryopreserved UCB cells and that this ability can be distinguished by profiling expression of certain sets of genes.
|
| 巻・号 |
397(4)
|
| ページ |
697-705
|
| 公開日 |
2010-7-9
|
| DOI |
10.1016/j.bbrc.2010.06.010
|
| PII |
S0006-291X(10)01113-7
|
| PMID |
20570655
|
| MeSH |
Animals
Antigens, CD34 / analysis
Bone Marrow
Cell Cycle / genetics
Cryopreservation
Fetal Blood / cytology*
Fetal Blood / metabolism*
Gene Expression Profiling*
Genetic Markers
Hematopoiesis / genetics
Hematopoiesis / physiology*
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells / metabolism
Hematopoietic Stem Cells / physiology*
Homeodomain Proteins / genetics
Humans
Mice
Mice, SCID
Transcription Factors / genetics
Transplantation, Heterologous
|
| IF |
2.985
|
| 引用数 |
2
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 研究用ヒト臍帯血幹細胞 |
|
