| RRC ID |
3832
|
| 著者 |
Takehana Y, Demiyah D, Naruse K, Hamaguchi S, Sakaizumi M.
|
| タイトル |
Evolution of different Y chromosomes in two medaka species, Oryzias dancena and O. latipes.
|
| ジャーナル |
Genetics
|
| Abstract |
Although the sex-determining gene DMY has been identified on the Y chromosome in the medaka (Oryzias latipes), this gene is absent in most Oryzias species, suggesting that closely related species have different sex-determining genes. Here, we investigated the sex-determination mechanism in O. dancena, which does not possess the DMY gene. Since heteromorphic sex chromosomes have not been reported in this species, a progeny test of sex-reversed individuals produced by hormone treatment was performed. Sex-reversed males yielded all-female progeny, indicating that O. dancena has an XX/XY sex-determination system. To uncover the cryptic sex chromosomes, sex-linked DNA markers were screened using expressed sequence tags (ESTs) established in O. latipes. Linkage analysis of isolated sex-linked ESTs showed a conserved synteny between the sex chromosomes in O. dancena and an autosome in O. latipes. Fluorescence in situ hybridization (FISH) analysis of these markers confirmed that sex chromosomes of these species are not homologous. These findings strongly suggest an independent origin of sex chromosomes in O. dancena and O. latipes. Further analysis of the sex-determining region in O. dancena should provide crucial insights into the evolution of sex-determination mechanisms in vertebrates.
|
| 巻・号 |
175(3)
|
| ページ |
1335-40
|
| 公開日 |
2007-3-1
|
| DOI |
10.1534/genetics.106.068247
|
| PII |
genetics.106.068247
|
| PMID |
17194774
|
| PMC |
PMC1840079
|
| MeSH |
Animals
Chromosome Mapping
Crosses, Genetic
Evolution, Molecular*
Expressed Sequence Tags
Female
Hermaphroditic Organisms
In Situ Hybridization, Fluorescence
Male
Oryzias / genetics*
Sex Determination Processes*
Species Specificity
Synteny / genetics
Y Chromosome / genetics*
|
| IF |
4.015
|
| 引用数 |
52
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| メダカ |
O. mekongensis
HdrR-II1 (IB178)
O. curvinotus
O. dancena (Chidambaram) (RS274)
O. dancena (Phuket) (RS275)
O. luzonensis (RS270) |
