論文 - 詳細
| RRC ID | 38322 |
|---|---|
| 著者 | Hebishima T, Matsumoto Y, Watanabe G, Soma G, Kohchi C, Taya K, Hayashi Y, Hirota Y. |
| タイトル | Protective effects of the immunopotentiator from Pantoea agglomerans 1 on chemotherapeutic agent-induced macrophage growth inhibition. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:The immunopotentiator from Pantoea agglomerans 1 (IP-PA1) is an edible lipopolysaccharide (LPS) derived from symbiotic bacteria found in crops. IP-PA1 is known to ameliorate chemotherapy-induced immunosuppression; therefore, its macrophage-activating effect in the presence of chemotherapeutic agents was evaluated. MATERIALS AND METHODS:Nuclear factor-kappaB (NF-kappaB) activation in IP-PA1-treated RAW264 and J774.1 cells was examined using Western blot analyses; Griess assay and ELISA were used to examine the production of nitric oxide and tumour necrosis factor alpha, respectively. The expression of apoptosis-related proteins was also assessed using Western blot analyses. The effect of IP-PA1 on doxorubicin-induced apoptosis was analyzed by flow cytometry after annexin-V staining. The growth of macrophages treated with chemotherapeutic agents and IP-PA1 was analyzed using an MTT assay. RESULTS:IP-PA1 activated NF-kappaB and ameliorated chemotherapy induced growth inhibition in the cells. CONCLUSION:IP-PA1 is an edible drug that can potentially support chemotherapy by ameliorating chemotherapy-induced immunosuppression. |
| 巻・号 | 30(6) |
| ページ | 2033-40 |
| 公開日 | 2010-6-1 |
| PII | 30/6/2033 |
| PMID | 20651348 |
| MeSH | Adjuvants, Immunologic / pharmacology* Animals Antineoplastic Agents / toxicity* Apoptosis / drug effects Cell Line Cell Proliferation / drug effects Macrophages / drug effects* Macrophages / physiology Mice NF-kappa B / metabolism Pantoea / chemistry* Proto-Oncogene Proteins c-bcl-2 / analysis bcl-X Protein / analysis |
| IF | 1.994 |
| 引用数 | 9 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |