論文 - 詳細
| RRC ID | 38330 |
|---|---|
| 著者 | Kasuya MC, Hatanaka K. |
| タイトル | Easy production of a glycolipid analogue using animal cells in culture. |
| ジャーナル | Chem Biodivers |
| Abstract |
A glycolipid analogue, GM4-type ganglioside, was obtained by a combination of chemical synthesis and biosynthetic processes in animal cells with dodecyl beta-D-galactoside (Gal C12) as primer. The primer was conveniently prepared in two steps: glycosylation, followed by deacetylation. The primer was introduced to mouse melanoma B16 cells to serve as substrate for cellular, enzyme-catalyzed glycosylation. Incubation of the cells in the presence of the primer resulted in sialylation of the galactose residue to afford a GM4 analogue that was released from the cells to the culture medium. The strategy of preparation of the GM4 analogue described in this study is a viable alternative to the existing methods. The saccharide-primer method is fast, convenient, not requiring expensive enzymes and glycosyl donors, and highly stereoselective. |
| 巻・号 | 7(2) |
| ページ | 440-6 |
| 公開日 | 2010-2-1 |
| DOI | 10.1002/cbdv.200900176 |
| PMID | 20151390 |
| MeSH | Animals Glycolipids / biosynthesis* Glycolipids / chemical synthesis* Glycolipids / chemistry Glycosylation Mice Tumor Cells, Cultured |
| IF | 2.039 |
| 引用数 | 2 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |