論文 - 詳細
| RRC ID | 38334 |
|---|---|
| 著者 | Kikuchi T, Nihei M, Nagai H, Fukushi H, Tabata K, Suzuki T, Akihisa T. |
| タイトル | Albanol A from the root bark of Morus alba L. induces apoptotic cell death in HL60 human leukemia cell line. |
| ジャーナル | Chem Pharm Bull (Tokyo) |
| Abstract |
Albanol A (1), isolated from the root bark extract of Morus alba (mulberry), was evaluated for the cytotoxic and apoptosis-inducing activities in human leukemia (HL60) cells, and for the inhibitory activity in human DNA topoisomerases (Topo) I and II. This compound showed potent cytotoxic activity (IC(50) 1.7 microM) on the cells, and potent inhibitory activity on topoisomerase II (IC(50) 22.8 microM). In addition, albanol A induced early apoptosis which was detected by observing the membrane phospholipid exposure in flow cytometry. Western blot analysis showed that albanol A markedly reduced the levels of procaspases-3, 8, and 9, while being increased the levels of cleaved caspases-3, 8, and 9. The Bax/Bcl-2 ratio was significantly increased by albanol A treatment. Furthermore, albanol A induced caspase-2 activation. These results suggested that albanol A induces apoptotic cell death in HL60 via both the cell death receptor pathway by stimulation of death receptor, and the mitochondrial pathway by Topo II inhibition through caspase-2 activation. Therefore, albanol A may be a promising lead compound for developing an effective drug for treatment of leukemia. |
| 巻・号 | 58(4) |
| ページ | 568-71 |
| 公開日 | 2010-4-1 |
| DOI | 10.1248/cpb.58.568 |
| PII | JST.JSTAGE/cpb/58.568 |
| PMID | 20410645 |
| MeSH | Antineoplastic Agents, Phytogenic / isolation & purification Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects Benzofurans / isolation & purification Benzofurans / pharmacology* Cell Line, Tumor DNA Topoisomerases, Type I / metabolism DNA Topoisomerases, Type II / metabolism Humans Leukemia / drug therapy* Morus / chemistry* Plant Roots / chemistry Terpenes / isolation & purification Terpenes / pharmacology* Topoisomerase I Inhibitors Topoisomerase II Inhibitors |
| IF | 1.416 |
| 引用数 | 59 |
| WOS 分野 | CHEMISTRY, MEDICINAL PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |