論文 - 詳細
| RRC ID | 38351 |
|---|---|
| 著者 | Mikami S, Kobayashi T, Imataka H. |
| タイトル | Cell-free protein synthesis systems with extracts from cultured human cells. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Cell-free protein synthesis systems have been established with extracts from cultured human cells, HeLa, and hybridoma cells. The former cell line is used to prepare extracts for robust translation, whereas the extract from the latter is primarily employed for expression of glycoproteins. Productivity of both systems can be enhanced by addition of K3L and GADD34, factors that diminish phosphorylation of eIF2alpha. The coupled transcription/translation system is also available as a convenient tool, particularly for the production of large recombinant proteins. |
| 巻・号 | 607 |
| ページ | 43-52 |
| 公開日 | 2010-1-1 |
| DOI | 10.1007/978-1-60327-331-2_5 |
| PMID | 20204847 |
| MeSH | Cell Extracts Cell-Free System HeLa Cells Humans Hybridomas Protein Biosynthesis* Protein Engineering* / methods RNA, Messenger / biosynthesis Recombinant Proteins / biosynthesis* Transcription, Genetic* |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HeLa.S3(RCB0191) HF10B4(RCB0708) |