論文 - 詳細
RRC ID | 38358 |
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著者 | Naito M, Vongsa S, Tsukune N, Ohashi A, Takahashi T. |
タイトル | Promyelocytic leukemia zinc finger mediates glucocorticoid-induced cell cycle arrest in the chondroprogenitor cell line ATDC5. |
ジャーナル | Mol Cell Endocrinol |
Abstract |
Glucocorticoids (GCs) affect the proliferation of growth plate chondrocytes. In this study, we investigated the role of the GC-inducible promyelocytic leukemia zinc finger (PLZF) gene in chondrocyte differentiation by using the chondrogenic cell line ATDC5. PLZF overexpression suppressed cell cycle progression (p < 0.01) and promoted differentiation into hypertrophic chondrocytes by inducing mRNA expression of alkaline phosphatase (p < 0.01), and the cyclin-dependent kinase (CDK) inhibitor p21 (p < 0.01). In contrast, PLZF knockdown impaired differentiation into hypertrophic chondrocytes and promoted cell cycle progression (p < 0.01). Treatment with the GC analogue dexamethasone (10(-6) M) suppressed cell cycle progression in ATDC5 cells. PLZF shRNA attenuated dexamethasone-induced cell cycle arrest (p < 0.01) by downregulating the mRNA expression of the CDK inhibitors p21 and p57 (p < 0.01). These results clearly indicated that PLZF promoted differentiation into hypertrophic chondrocytes and mediated dexamethasone-induced cell cycle arrest by regulating CDK inhibitors. |
巻・号 | 417 |
ページ | 114-23 |
公開日 | 2015-12-5 |
DOI | 10.1016/j.mce.2015.09.026 |
PII | S0303-7207(15)30092-7 |
PMID | 26419928 |
MeSH | Alkaline Phosphatase / genetics Animals Cell Cycle Checkpoints / drug effects* Cell Differentiation / drug effects Cell Line Chondrocytes / cytology Chondrocytes / drug effects* Cyclin-Dependent Kinase Inhibitor p21 / genetics Dexamethasone / pharmacology Gene Expression Regulation / drug effects Glucocorticoids / pharmacology* Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism* Mice Promyelocytic Leukemia Zinc Finger Protein |
IF | 3.871 |
引用数 | 5 |
WOS 分野 | ENDOCRINOLOGY & METABOLISM CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | ATDC5(RCB0565) |