RRC ID 38358
著者 Naito M, Vongsa S, Tsukune N, Ohashi A, Takahashi T.
タイトル Promyelocytic leukemia zinc finger mediates glucocorticoid-induced cell cycle arrest in the chondroprogenitor cell line ATDC5.
ジャーナル Mol Cell Endocrinol
Abstract Glucocorticoids (GCs) affect the proliferation of growth plate chondrocytes. In this study, we investigated the role of the GC-inducible promyelocytic leukemia zinc finger (PLZF) gene in chondrocyte differentiation by using the chondrogenic cell line ATDC5. PLZF overexpression suppressed cell cycle progression (p < 0.01) and promoted differentiation into hypertrophic chondrocytes by inducing mRNA expression of alkaline phosphatase (p < 0.01), and the cyclin-dependent kinase (CDK) inhibitor p21 (p < 0.01). In contrast, PLZF knockdown impaired differentiation into hypertrophic chondrocytes and promoted cell cycle progression (p < 0.01). Treatment with the GC analogue dexamethasone (10(-6) M) suppressed cell cycle progression in ATDC5 cells. PLZF shRNA attenuated dexamethasone-induced cell cycle arrest (p < 0.01) by downregulating the mRNA expression of the CDK inhibitors p21 and p57 (p < 0.01). These results clearly indicated that PLZF promoted differentiation into hypertrophic chondrocytes and mediated dexamethasone-induced cell cycle arrest by regulating CDK inhibitors.
巻・号 417
ページ 114-23
公開日 2015-12-5
DOI 10.1016/j.mce.2015.09.026
PII S0303-7207(15)30092-7
PMID 26419928
MeSH Alkaline Phosphatase / genetics Animals Cell Cycle Checkpoints / drug effects* Cell Differentiation / drug effects Cell Line Chondrocytes / cytology Chondrocytes / drug effects* Cyclin-Dependent Kinase Inhibitor p21 / genetics Dexamethasone / pharmacology Gene Expression Regulation / drug effects Glucocorticoids / pharmacology* Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism* Mice Promyelocytic Leukemia Zinc Finger Protein
IF 3.871
引用数 5
WOS 分野 ENDOCRINOLOGY & METABOLISM CELL BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)