| RRC ID |
38362
|
| 著者 |
Ohta T, Kusano K, Ido A, Miura C, Miura T.
|
| タイトル |
Silkrose: A novel acidic polysaccharide from the silkmoth that can stimulate the innate immune response.
|
| ジャーナル |
Carbohydr Polym
|
| Abstract |
We have identified a novel acidic polysaccharide from silkmoth (Antheraea yamamai) pupae that activates the mammalian innate immune response. This bioactive polysaccharide was isolated using nitric oxide production in mouse RAW264 macrophages as an indicator of immunostimulatory activity. We named this polysaccharide "silkrose". It has a molecular weight of 3.15 × 10(5) and comprises nine monosaccharides. The expression profiles indicated that silkrose induced the expression of proinflammatory cytokines and interferon β that exist downstream of MyD88-dependent and MyD88-indeptendent signaling pathways. Also, the inhibition of Toll-like receptor 4 (TLR4), which exists upstream of the signaling pathways, led to the suppression of NO production by silkrose. Furthermore, this polysaccharide promoted the activation of nuclear factor kappa B in RAW264 cells, indicating that it stimulates the induction of various cytokines in macrophages through the TLR4 signaling pathway. Our results thus suggest that silkrose activates the innate immune response to various pathogenic microorganisms and viral infections.
|
| 巻・号 |
136
|
| ページ |
995-1001
|
| 公開日 |
2016-1-20
|
| DOI |
10.1016/j.carbpol.2015.09.070
|
| PII |
S0144-8617(15)00935-2
|
| PMID |
26572439
|
| MeSH |
Animals
Bombyx / chemistry*
Cell Line
Cytokines / genetics
Cytokines / metabolism
Immunity, Innate*
Immunologic Factors / chemistry*
Immunologic Factors / pharmacology
Macrophages / drug effects
Macrophages / immunology
Mice
Polysaccharides / chemistry*
Polysaccharides / pharmacology
Toll-Like Receptor 4 / genetics
Toll-Like Receptor 4 / metabolism
|
| IF |
7.182
|
| 引用数 |
14
|
|
WOS 分野
|
CHEMISTRY, APPLIED
CHEMISTRY, ORGANIC
POLYMER SCIENCE
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
