論文 - 詳細
RRC ID | 38390 |
---|---|
著者 | Tan W, Lim SG, Tan TM. |
タイトル | Up-regulation of microRNA-210 inhibits proliferation of hepatocellular carcinoma cells by targeting YES1. |
ジャーナル | World J Gastroenterol |
Abstract |
AIM:To determine the expression of microRNA-210 (miR-210) in hepatocellular carcinoma (HCC) and to examine its role using HCC cells. METHODS:The expression of miR-210 was determined in 21 pairs of HCC samples and the corresponding surrounding non-tumor tissues. The effects of miR-210 on proliferation and cell cycle progression were examined using HepG2 and HuH7 cells. Over-expression and inhibition of miR-210 was achieved by transfection of the cells with miR-210 mimic or inhibitor. Luciferase reporter constructs were used to identify the miR-210 interacting site on Yes1. Yes1 expression was examined after miR-210 transfection, as well as in the HCC samples. RESULTS:miR-210 was significantly up-regulated by 3.4 fold (P < 0.01) in the tumor samples. The over-expression of miR-210 significantly reduced cell proliferation compared to the mock-treated cells (68.9% ± 7.4% and 53.6% ± 5.0%, P < 0.05 for the HepG2 and HuH7 cells respectively). Analysis of the HuH7 cells transfected with miR-210 mimic by flow cytometry showed that the cells took a longer time to reach the G2/M phase. The interaction between miR-210 and the 3'UTR of the Yes1 transcript was confirmed using a luciferase reporter assay. Over-expression of miR-210 reduced the expression of Yes1 protein in both HuH7 and HepG2 cells. Tumors with a greater than four-fold increase in the expression of miR-210 showed consistently lower expressions of Yes1 in the tumors. In nocodazole-treated cells with a significant G2/M cell population, Yes1 protein was significantly reduced and pre-inhibition of miR-210 in HuH7 cells was able to prevent the reduction of Yes1 protein expression. Knock-down of Yes1 by siRNA also led to reduced cell proliferation (70.8% ± 7.5%, P < 0.05 in the HuH7 cells). CONCLUSION:Up-regulation of miR-210 inhibits cell proliferation. Yes1 is a target of miR-210 and affects cell proliferation in HCC. |
巻・号 | 21(46) |
ページ | 13030-41 |
公開日 | 2015-12-14 |
DOI | 10.3748/wjg.v21.i46.13030 |
PMID | 26676187 |
PMC | PMC4674721 |
MeSH | 3' Untranslated Regions Adult Aged Binding Sites Carcinoma, Hepatocellular / enzymology* Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / pathology Cell Proliferation* Female G2 Phase Cell Cycle Checkpoints Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Hep G2 Cells Humans Liver Neoplasms / enzymology* Liver Neoplasms / genetics Liver Neoplasms / pathology Male MicroRNAs / genetics MicroRNAs / metabolism* Middle Aged Proto-Oncogene Proteins c-yes / genetics Proto-Oncogene Proteins c-yes / metabolism* RNA Interference Signal Transduction Time Factors Transfection |
IF | 3.665 |
引用数 | 15 |
WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
リソース情報 | |
ヒト・動物細胞 | HuH-7 |