| RRC ID |
38407
|
| 著者 |
Myochin T, Kiyose K, Hanaoka K, Kojima H, Terai T, Nagano T.
|
| タイトル |
Rational design of ratiometric near-infrared fluorescent pH probes with various pKa values, based on aminocyanine.
|
| ジャーナル |
J Am Chem Soc
|
| Abstract |
Novel ratiometric, near-infrared fluorescent pH probes with various pK(a) values have been designed and synthesized on the basis of aminocyanine bearing a diamine moiety, and their photochemical properties were evaluated. Under acidic conditions, these pH probes showed a 46- to 83-nm red shift of the absorption maximum. This change is sufficiently large to permit their use as ratiometric pH probes, and is reversible, whereas monoamine-substituted aminocyanines showed irreversible changes because of their instability under acidic conditions. Furthermore, the pK(a) values of these probes can be predicted from the calculated pK(a) values of the diamine moieties, obtained from the SciFinder database. This design strategy is very simple and flexible, and should be applicable to develop NIR pH probes for various applications.
|
| 巻・号 |
133(10)
|
| ページ |
3401-9
|
| 公開日 |
2011-3-16
|
| DOI |
10.1021/ja1063058
|
| PMID |
21341656
|
| MeSH |
Diamines / chemistry*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Hydrogen-Ion Concentration
Spectroscopy, Near-Infrared / methods*
|
| IF |
14.612
|
| 引用数 |
203
|
|
WOS 分野
|
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
|
