論文 - 詳細
| RRC ID | 38424 |
|---|---|
| 著者 | Yamada Y, Harashima H. |
| タイトル | Delivery of bioactive molecules to the mitochondrial genome using a membrane-fusing, liposome-based carrier, DF-MITO-Porter. |
| ジャーナル | Biomaterials |
| Abstract |
Mitochondrial dysfunction has been implicated in a variety of human diseases. It is now well accepted that mutations and defects in the mitochondrial genome form the basis of these diseases. Therefore, mitochondrial gene therapy and diagnosis would be expected to have great medical benefits. To achieve such a strategy, it will be necessary to deliver therapeutic agents into mitochondria in living cells. We report here on an approach to accomplish this via the use of a Dual Function (DF)-MITO-Porter, aimed at the mitochondrial genome, so-called mitochondrial DNA (mtDNA). The DF-MITO-Porter, a nano carrier for mitochondrial delivery, has the ability to penetrate the endosomal and mitochondrial membranes via step-wise membrane fusion. We first constructed a DF-MITO-Porter encapsulating DNase I protein as a bioactive cargo. It was expected that mtDNA would be digested, when the DNase I was delivered to the mitochondria. We observed the intracellular trafficking of the carriers, and then measured mitochondrial activity and mtDNA-levels after the delivery of DNase I by the DF-MITO-Porter. The findings confirm that the DF-MITO-Porter effectively delivered the DNase I into the mitochondria, and provides a demonstration of its potential use in therapies that are selective for the mitochondrial genome. |
| 巻・号 | 33(5) |
| ページ | 1589-95 |
| 公開日 | 2012-2-1 |
| DOI | 10.1016/j.biomaterials.2011.10.082 |
| PII | S0142-9612(11)01315-9 |
| PMID | 22105068 |
| MeSH | Cell Nucleus / genetics DNA, Mitochondrial / genetics Deoxyribonuclease I / metabolism* Drug Carriers / chemistry* Drug Delivery Systems / methods* Enzymes, Immobilized / metabolism Genome, Mitochondrial* HeLa Cells Humans Intracellular Space / metabolism Liposomes / chemistry* Membrane Fusion* Microscopy, Confocal Mitochondria / metabolism Nanoparticles / chemistry* Nanostructures / chemistry |
| IF | 10.317 |
| 引用数 | 40 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |