論文 - 詳細
| RRC ID | 38461 |
|---|---|
| 著者 | Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. |
| タイトル | Cy5-conjugated hybridization-sensitive fluorescent oligonucleotides for ratiometric analysis of nuclear poly(A)+ RNA. |
| ジャーナル | Bioconjug Chem |
| Abstract |
Subnuclear poly(A)(+) RNA localization in living mammalian cells was visualized by ratiometric analysis using hybridization-sensitive fluorescent oligonucleotide probes. Probes were oligonucleotides, which contained a Cy5 fluorescent dye at the strand end and a thiazole orange double-labeled nucleotide inside strand. A ratiometric analysis using poly(A)-targeting probes revealed a distribution of the probe itself as red fluorescence and localization of the target RNA sequence in cell nuclei as green fluorescence. The fluorescence of the subnuclear poly(A)(+) RNA hybridized with the poly(A)-targeting probes was observed as puncta in interchromatin areas. |
| 巻・号 | 22(8) |
| ページ | 1625-30 |
| 公開日 | 2011-8-17 |
| DOI | 10.1021/bc200184a |
| PMID | 21755986 |
| MeSH | Benzothiazoles Carbocyanines* Fluorescent Dyes / chemistry* Nucleic Acid Hybridization Oligonucleotide Probes / chemistry* Poly A Quinolines RNA / analysis* |
| IF | 4.031 |
| 引用数 | 9 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |