論文 - 詳細
| RRC ID | 38474 |
|---|---|
| 著者 | Kobayashi S, Taki T, Chinen Y, Tsutsumi Y, Ohshiro M, Kobayashi T, Matsumoto Y, Kuroda J, Horiike S, Nishida K, Taniwaki M. |
| タイトル | Identification of IGHCδ-BACH2 fusion transcripts resulting from cryptic chromosomal rearrangements of 14q32 with 6q15 in aggressive B-cell lymphoma/leukemia. |
| ジャーナル | Genes Chromosomes Cancer |
| Abstract |
In B-cell malignancies, genes implicated in B-cell differentiation, germinal center formation, apoptosis, and cell cycle regulation are juxtaposed to immunoglobulin loci through chromosomal translocations. In this study, we identified the BTB and CNC homology 2 (BACH2) gene as a novel translocation partner of the immunoglobulin heavy chain (IGH) locus in a patient with IGH-MYC-positive, highly aggressive B-cell lymphoma/leukemia carrying der(14)t(8;14) and del(6)(q15). Fluorescence in situ hybridization analysis using an IGH/MYC probe detected an IGH-MYC fusion signal on der(14) and IGH signal on del(6). Genome copy number analysis showed a deletion in the 6q15-25 region and a centromeric breakpoint within the BACH2 gene. cDNA bubble polymerase chain reaction using BACH2 primers revealed that the first exon of Cδ was fused to the 5'-untranslated region of BACH2 exon 2. The Cδ-BACH2 fusion transcript consisted of exon 1 of Cδ and exons 2 to 9 of BACH2, encompassing the entire BACH2 coding region, and the BACH2 was highly expressed in this patient. These results indicate that Cδ-BACH2 fusion may cause constitutive activation of BACH2. Although additional screening of 47 samples of B-cell non-Hodgkin's lymphoma (B-NHL) patients and 29 cell lines derived from B-cell malignancies by double-color fluorescence in situ hybridization analysis detected a split signal with deletion of centromeric region of BACH2 only in a patient with follicular lymphoma, BACH2 was highly expressed in lymphoma cells of the patient and B-NHL cell lines with IGH-MYC translocation. These findings suggest that BACH2 plays a critical role in B-cell lymphomagenesis, especially related to IGH-MYC translocation in some way. |
| 巻・号 | 50(4) |
| ページ | 207-16 |
| 公開日 | 2011-4-1 |
| DOI | 10.1002/gcc.20845 |
| PMID | 21319257 |
| MeSH | Aged B-Lymphocytes / metabolism Base Sequence Basic-Leucine Zipper Transcription Factors / genetics* Chromosome Breakpoints Chromosomes, Human, Pair 14 / genetics* Chromosomes, Human, Pair 6 / genetics* DNA, Neoplasm / genetics Gene Expression Regulation, Leukemic Gene Fusion Genes, myc Humans Immunoglobulin delta-Chains / genetics* In Situ Hybridization, Fluorescence Leukemia / genetics Lymphoma, B-Cell / genetics* Lymphoma, B-Cell / pathology Male Molecular Sequence Data Sequence Analysis, DNA Translocation, Genetic* |
| IF | 3.444 |
| 引用数 | 28 |
| WOS 分野 | GENETICS & HEREDITY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |