RRC ID 38530
著者 Qin XY, Wei F, Yoshinaga J, Yonemoto J, Tanokura M, Sone H.
タイトル siRNA-mediated knockdown of aryl hydrocarbon receptor nuclear translocator 2 affects hypoxia-inducible factor-1 regulatory signaling and metabolism in human breast cancer cells.
ジャーナル FEBS Lett
Abstract Recent human studies found that the mRNA expression level of aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2) was positively associated with the prognosis of breast cancer. In this study, we used small interfering RNA techniques to knockdown ARNT2 expression in MCF7 human breast cancer cells, and found that an almost 40% downregulation of ARNT2 mRNA expression increased the expression of sensitive to apoptosis gene (3.36-fold), and decreased the expression of von Hippel-Lindau (0.27-fold) and matrix metalloproteinase-1 (0.35-fold). The metabolite analysis revealed the contents of glucose, glycine, betaine, phosphocholine, pyruvate and lactate involved in the hypoxia-inducible factor (HIF)-1-dependent glycolytic pathway were significantly lower in cells treated with siARNT2. Our results suggested that ARNT2 might play an important role in the modulation of HIF-1-regulated signaling and metabolism.
巻・号 585(20)
ページ 3310-5
公開日 2011-10-20
DOI 10.1016/j.febslet.2011.09.017
PII S0014-5793(11)00696-X
PMID 21945317
MeSH Aryl Hydrocarbon Receptor Nuclear Translocator / biosynthesis* Aryl Hydrocarbon Receptor Nuclear Translocator / genetics Basic Helix-Loop-Helix Transcription Factors / biosynthesis* Basic Helix-Loop-Helix Transcription Factors / genetics Breast Neoplasms / genetics Breast Neoplasms / metabolism* Cell Line, Tumor Female Gene Expression Regulation, Neoplastic* Gene Knockdown Techniques Humans Hypoxia-Inducible Factor 1 / genetics Hypoxia-Inducible Factor 1 / metabolism Neoplasm Proteins / biosynthesis* Neoplasm Proteins / genetics RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Signal Transduction*
IF 3.057
引用数 16
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 MCF7(RCB1904)