論文 - 詳細
| RRC ID | 38533 |
|---|---|
| 著者 | Senju S, Haruta M, Matsumura K, Matsunaga Y, Fukushima S, Ikeda T, Takamatsu K, Irie A, Nishimura Y. |
| タイトル | Generation of dendritic cells and macrophages from human induced pluripotent stem cells aiming at cell therapy. |
| ジャーナル | Gene Ther |
| Abstract |
This report describes generation of dendritic cells (DCs) and macrophages from human induced pluripotent stem (iPS) cells. iPS cell-derived DC (iPS-DC) exhibited the morphology of typical DC and function of T-cell stimulation and antigen presentation. iPS-DC loaded with cytomegalovirus (CMV) peptide induced vigorous expansion of CMV-specific autologous CD8+ T cells. Macrophages (iPS-MP) with activity of zymosan phagocytosis and C5a-induced chemotaxis were also generated from iPS cells. Genetically modified iPS-MPs were generated by the introduction of expression vectors into undifferentiated iPS cells, isolation of transfectant iPS cell clone and subsequent differentiation. By this procedure, we generated iPS-MP expressing a membrane-bound form of single chain antibody (scFv) specific to amyloid β (Aβ), the causal protein of Alzheimer's disease. The scFv-transfectant iPS-MP exhibited efficient Aβ-specific phagocytosis activity. iPS-MP expressing CD20-specific scFv engulfed and killed BALL-1 B-cell leukemia cells. Anti-BALL-1 effect of iPS-MP in vivo was demonstrated in a xeno-transplantation model using severe combined immunodeficient mice. In addition, we established a xeno-free culture protocol to generate iPS-DC and iPS-MP. Collectively, we demonstrated the possibility of application of iPS-DC and macrophages to cell therapy. |
| 巻・号 | 18(9) |
| ページ | 874-83 |
| 公開日 | 2011-9-1 |
| DOI | 10.1038/gt.2011.22 |
| PII | gt201122 |
| PMID | 21430784 |
| MeSH | Cell Differentiation Cell Line, Tumor Cell- and Tissue-Based Therapy / methods* Dendritic Cells / cytology* Humans Induced Pluripotent Stem Cells / cytology* Leukemia, B-Cell / immunology Lymphocyte Activation Macrophages / cytology* Phagocytosis Transfection |
| IF | 4.128 |
| 引用数 | 64 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MEDICINE, RESEARCH & EXPERIMENTAL BIOCHEMISTRY & MOLECULAR BIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 40 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BALL-1(RCB0256) OP9(RCB1124) |