RRC ID 38534
著者 Kitagawa J, Hara T, Tsurumi H, Ninomiya S, Ogawa K, Adachi S, Kanemura N, Kasahara S, Shimizu M, Moriwaki H.
タイトル Synergistic growth inhibition in HL-60 cells by the combination of acyclic retinoid and vitamin K2.
ジャーナル J Cancer Res Clin Oncol
Abstract PURPOSE:The aim of this study was to assess the effects of acyclic retinoid (ACR) and vitamin K(2) (VK(2)) in HL-60 cells.
METHODS:We used HL-60 cells, and the Trypan Blue dye exclusion method was used for cell proliferation assays. For detection of apoptosis, the Annexin V-binding capacity of treated cells was examined by flow cytometry. To evaluate the cell cycle, we used a FITC BrdU Flow KIT and flow cytometry. Total extracted and equivalent amounts of protein were examined by Western blotting using specific antibodies.
RESULTS:ACR and VK(2) dose dependently inhibited the proliferation of HL-60 cells. These two agents in combination synergistically inhibited cell growth and induced apoptosis. VK(2) inhibited activation of the Ras/MAPK signaling pathway, and ACR plus VK(2) cooperatively inhibited phosphorylation of RXRα and the growth of HL-60 cells. Moreover, ACR and VK(2) induced increases in G0/G1 phase HL-60 cells, alone and synergistically in combination.
CONCLUSION:The synergistic effects of ACR and VK(2) on HL-60 cells may provide a novel strategy for treating leukemia.
巻・号 137(5)
ページ 779-87
公開日 2011-5-1
DOI 10.1007/s00432-010-0938-0
PMID 20676681
MeSH Apoptosis / drug effects Blotting, Western Cell Proliferation / drug effects* Drug Synergism Extracellular Signal-Regulated MAP Kinases / physiology HL-60 Cells Humans Retinoid X Receptor alpha / physiology Retinoids / administration & dosage Retinoids / pharmacology* Vitamin K 2 / administration & dosage Vitamin K 2 / pharmacology*
IF 3.656
引用数 9
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 HL60(RCB0041)