論文 - 詳細
RRC ID | 38534 |
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著者 | Kitagawa J, Hara T, Tsurumi H, Ninomiya S, Ogawa K, Adachi S, Kanemura N, Kasahara S, Shimizu M, Moriwaki H. |
タイトル | Synergistic growth inhibition in HL-60 cells by the combination of acyclic retinoid and vitamin K2. |
ジャーナル | J Cancer Res Clin Oncol |
Abstract |
PURPOSE:The aim of this study was to assess the effects of acyclic retinoid (ACR) and vitamin K(2) (VK(2)) in HL-60 cells. METHODS:We used HL-60 cells, and the Trypan Blue dye exclusion method was used for cell proliferation assays. For detection of apoptosis, the Annexin V-binding capacity of treated cells was examined by flow cytometry. To evaluate the cell cycle, we used a FITC BrdU Flow KIT and flow cytometry. Total extracted and equivalent amounts of protein were examined by Western blotting using specific antibodies. RESULTS:ACR and VK(2) dose dependently inhibited the proliferation of HL-60 cells. These two agents in combination synergistically inhibited cell growth and induced apoptosis. VK(2) inhibited activation of the Ras/MAPK signaling pathway, and ACR plus VK(2) cooperatively inhibited phosphorylation of RXRα and the growth of HL-60 cells. Moreover, ACR and VK(2) induced increases in G0/G1 phase HL-60 cells, alone and synergistically in combination. CONCLUSION:The synergistic effects of ACR and VK(2) on HL-60 cells may provide a novel strategy for treating leukemia. |
巻・号 | 137(5) |
ページ | 779-87 |
公開日 | 2011-5-1 |
DOI | 10.1007/s00432-010-0938-0 |
PMID | 20676681 |
MeSH | Apoptosis / drug effects Blotting, Western Cell Proliferation / drug effects* Drug Synergism Extracellular Signal-Regulated MAP Kinases / physiology HL-60 Cells Humans Retinoid X Receptor alpha / physiology Retinoids / administration & dosage Retinoids / pharmacology* Vitamin K 2 / administration & dosage Vitamin K 2 / pharmacology* |
IF | 3.656 |
引用数 | 9 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | HL60(RCB0041) |