| RRC ID |
38554
|
| 著者 |
Doi T, Maniwa Y, Tanaka Y, Tane S, Hashimoto S, Ohno Y, Nishio W, Nishimura Y, Ohbayashi C, Okita Y, Hayashi Y, Yoshimura M.
|
| タイトル |
MT1-MMP plays an important role in an invasive activity of malignant pleural mesothelioma cell.
|
| ジャーナル |
Exp Mol Pathol
|
| Abstract |
Malignant pleural mesothelioma (MPM) has a poor prognosis and is a treatment resistant tumor, which is increasing in frequency throughout the world. The poor prognosis is due to the aggressive local invasiveness rather than distant metastasis. In this study, we established a cell line of malignant mesothelioma from a clinical specimen and assessed the relationship between the expression of MT1-MMP and the invasion ability of that line, as well as the cultured cells of several other lines, using the simple method that we created previously. We established a cell line from a clinical specimen from a patient with malignant mesothelioma. We assessed the invasive activities of MPM cells in an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) system that enabled us to visualize cell movements during invasion. To assess the role of MT1-MMP in the invasive activity of MPM cells, we knocked down its expression by RNA interference (RNAi). The invasion assay with DL-CGH revealed that a high expression of MT1-MMP in MPM cells was associated with aggressive invasive activity. The RNAi of MT1-MMP indicated that the expression of MT1-MMP might have a crucial role in the invasiveness of MPM cells. The MT1-MMP expression in MPM cells is related to their capacity for locally aggressive spreading into the pleura and the surrounding tissues, and MT1-MMP should be a suitable molecular target for the suppression of the invasiveness of MPM.
|
| 巻・号 |
90(1)
|
| ページ |
91-6
|
| 公開日 |
2011-2-1
|
| DOI |
10.1016/j.yexmp.2010.10.008
|
| PII |
S0014-4800(10)00137-1
|
| PMID |
20969861
|
| MeSH |
Cell Line, Tumor
Cell Movement / genetics
Gene Knockdown Techniques
Humans
Matrix Metalloproteinase 14 / genetics
Matrix Metalloproteinase 14 / physiology*
Mesothelioma / genetics*
Neoplasm Invasiveness / pathology
Pleural Neoplasms / genetics*
RNA Interference
|
| IF |
2.28
|
| 引用数 |
11
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
WI-38(RCB0704) |
