論文 - 詳細
| RRC ID | 38561 |
|---|---|
| 著者 | Nishiura H, Zhao R, Yamamoto T. |
| タイトル | The role of the ribosomal protein S19 C-terminus in altering the chemotaxis of leucocytes by causing functional differences in the C5a receptor response. |
| ジャーナル | J Biochem |
| Abstract |
Ribosomal protein S19 (RP S19) oligomers have been discovered as the first chemoattractant of migrating monocytes/macrophages to apoptotic cells via the C5a receptor (C5aR). In contrast to C5a, a fusion of the C-terminus (I(134)-H(145)) of RP S19 to C5a, the C5a/RP S19 chimera, substitutes for the RP S19 oligomers and is able to replicate C5aR antagonist-induced and agonist-induced dual effects on neutrophil and monocyte chemotactic responses, respectively. We recently discovered a gain of binding affinity when the I(134)-H(145) inhibited the activation of neutrophil C5aR-mediated chemotactic pathways. However, the opposing ligand-dependent chemotactic mechanisms are not fully understood. In this study, a loss of this additional binding affinity appeared to cause the monocyte C5aR to activate an alternative signalling pathway. The p38 mitogen activated-protein kinase (MAPK) pathway was linked to cell migration rather than a classical extracellular-regulated kinase 1/2 pathway commonly used by C5a. C5aR internalization was not involved in the alternative chemotactic pathway. We propose a model of activation involving a C5aR co-molecule that interferes with the C5aR-Gi protein interaction upon binding to the I(134)-H(145) in neutrophils; however, a free I(134)-H(145) from the C5aR co-molecule can guide the alternative activation of the chemotactic p38MAPK pathway in monocytes/macrophages. |
| 巻・号 | 150(3) |
| ページ | 271-7 |
| 公開日 | 2011-9-1 |
| DOI | 10.1093/jb/mvr067 |
| PII | mvr067 |
| PMID | 21613290 |
| MeSH | Cell Line, Tumor Chemotactic Factors / metabolism Chemotaxis, Leukocyte* Complement C5a / chemistry Complement C5a / metabolism Humans Macrophages / physiology Mast Cells / cytology Mast Cells / metabolism Monocytes / physiology* Neutrophils / physiology Protein Binding Receptor, Anaphylatoxin C5a / metabolism* Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Signal Transduction p38 Mitogen-Activated Protein Kinases / chemistry p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 2.476 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |