| RRC ID |
38580
|
| Author |
Kihara T, Shinohara S, Fujikawa R, Sugimoto Y, Murata M, Miyake J.
|
| Title |
Regulation of cysteine-rich protein 2 localization by the development of actin fibers during smooth muscle cell differentiation.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Cysteine-rich protein 2 (CRP2) is a cofactor for smooth muscle cell (SMC) differentiation. Here, we examined the mechanism of CRP2 distribution dynamics during SMC differentiation. CRP2 protein directly associated with F-actin through its N-terminal LIM domain and Gly-rich region, as determined by ELISA. In undifferentiated cells that contain few actin stress fibers, CRP2 was broadly distributed throughout the whole cell, including the nucleus. After induction of SMC differentiation, CRP2 localized to actin stress fibers as they formed. The stress fiber-localized CRP2 entered the nucleus because of induced actin depolymerization. These CRP2 dynamics were reproduced by in silico simulation. CRP2 localization dynamics, which affect CRP2 function, are regulated by the formation of actin stress fibers in conjunction with SMC differentiation.
|
| Volume |
411(1)
|
| Pages |
96-101
|
| Published |
2011-7-22
|
| DOI |
10.1016/j.bbrc.2011.06.100
|
| PII |
S0006-291X(11)01080-1
|
| PMID |
21718689
|
| MeSH |
Actins / metabolism*
Animals
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Differentiation*
Cell Line
LIM Domain Proteins
Mice
Myocytes, Smooth Muscle / cytology*
Myocytes, Smooth Muscle / metabolism
Photobleaching
Stress Fibers / metabolism*
|
| IF |
2.985
|
| Times Cited |
12
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
