Reference - Detail
| RRC ID | 3859 |
|---|---|
| Author | Ito T, Maki N, Hazeki O, Sasaki K, Nekooki M. |
| Title | Extracellular and transmembrane region of a podocalyxin-like protein 1 fragment identified from colon cancer cell lines. |
| Journal | Cell Biol Int |
| Abstract |
Regulated intramembrane proteolysis of membrane proteins has been shown to play an important role in cell differentiation and in the pathogenesis of diseases. The aim of the present study was to identify novel peptides generated by intramembrane proteolysis. The peptides were identified in serum-free cultured (SFC) media from various cell lines by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). A 2315-Da peptide found only in medium from SFC colon cancer cell lines was identified and shown to consist of a portion of both the extracellular and transmembrane regions of human podocalyxin-like 1. This protein fragment was not found in lung or pancreatic cancer cell lines by immunoprecipitation-SELDI tests using an antibody specific to this fragment, suggesting that this human podocalyxin-like protein 1 fragment may be unique to colon cancer cell lines. |
| Volume | 31(12) |
| Pages | 1518-24 |
| Published | 2007-12-1 |
| DOI | 10.1016/j.cellbi.2007.06.017 |
| PII | S1065-6995(07)00190-4 |
| PMID | 17719804 |
| MeSH | Amino Acid Sequence Animals Biomarkers, Tumor / isolation & purification* Cell Line, Tumor Colonic Neoplasms / chemistry* Colonic Neoplasms / metabolism Culture Media, Serum-Free / analysis Humans Membrane Proteins / isolation & purification* Membrane Proteins / metabolism Mice Mice, Inbred BALB C Neoplasm Proteins / isolation & purification* Peptide Mapping* Protein Structure, Tertiary Sialoglycoproteins / isolation & purification* Sialoglycoproteins / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods |
| IF | 2.571 |
| Times Cited | 5 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CACO-2(RCB0988) LC-2/ad(RCB0440) |