論文 - 詳細
| RRC ID | 38601 |
|---|---|
| 著者 | Miyazawa K, He F, Kawase M, Kubota A, Yoda K, Hiramatsu M. |
| タイトル | Enhancement of immunoregulatory effects of Lactobacillus gasseri TMC0356 by heat treatment and culture medium. |
| ジャーナル | Lett Appl Microbiol |
| Abstract |
AIMS:The aim of this study was to investigate the influence of heat treatment and culture media on the immunoregulatory effects of a probiotic strain, Lactobacillus gasseri TMC0356 (TMC0356). METHODS AND RESULTS:TMC0356 cultured in deMan-Rogosa-Sharpe and same food grade (FG) media were inactivated with the heat treatment at 70 and 90°C. Viable and heat-killed TMC0356 were tested for their ability to induce interleukin (IL)-12 production in the murine macrophage cell line J774.1. These TMC0356 were examined for their resistance to N-acetylmuramidase. Their morphology was observed by scanning electron microscopy. The heat-killed TMC0356 significantly induced IL-12 production in J774.1 cells and exhibited enhanced resistance to N-acetylmuramidase compared with viable TMC0356. Morphological changes were observed in TMC0356 when cultured in FG medium. Cell morphology and induction of IL-12 production in J774.1 cells were also associated. CONCLUSIONS:These results suggest that heat treatment and culture medium composition modified the immunoregulatory effects of TMC0356 to induce IL-12 production in macrophages. SIGNIFICANCE AND IMPACT OF THE STUDY:These results demonstrate that probiotic immunoregulatory effects may be modified by the processing technology of cell preparation. |
| 巻・号 | 53(2) |
| ページ | 210-6 |
| 公開日 | 2011-8-1 |
| DOI | 10.1111/j.1472-765X.2011.03093.x |
| PMID | 21623846 |
| MeSH | Animals Cell Line Culture Media / metabolism* Glycoside Hydrolases / toxicity Hot Temperature Interleukin-12 / metabolism Lactobacillus / growth & development* Macrophages / metabolism Mice Mice, Inbred BALB C Microbial Viability Probiotics / pharmacology* |
| IF | 2.173 |
| 引用数 | 7 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | J774.1 |