Reference - Detail
| RRC ID | 38605 |
|---|---|
| Author | Kobayashi T, Nakamura Y, Mikami S, Masutani M, Machida K, Imataka H. |
| Title | Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube. |
| Journal | Biotechnol Lett |
| Abstract |
Virus particles are used in vaccination, drug delivery, and material sciences. Here we devised a system where the RNA virus encephalomyocarditis virus (EMCV) is synthesized from DNA templates in vitro. When a plasmid or a PCR product harboring the full-length cDNA of EMCV in the T7 promoter/terminator unit was incubated in a HeLa cell extract supplemented with T7 RNA polymerase, EMCV was produced within 4 h at an efficiency of over 10-fold compared with the system programmed with the EMCV RNA. The EMCV RNA transcribed by the virally encoded RNA-dependent RNA polymerase was predominantly incorporated into the EMCV particle even in the presence of a larger amount of the EMCV RNA transcribed by T7 RNA polymerase from the plasmid. |
| Volume | 34(1) |
| Pages | 67-73 |
| Published | 2012-1-1 |
| DOI | 10.1007/s10529-011-0744-z |
| PMID | 21952914 |
| MeSH | Biotechnology / methods Cell Extracts Cell-Free System Encephalomyocarditis virus / genetics* HeLa Cells Humans Plasmids RNA, Viral / biosynthesis* Viral Proteins / biosynthesis* Virology / methods Virus Assembly* |
| IF | 1.977 |
| Times Cited | 8 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Human and Animal Cells | HeLa S3 |