論文 - 詳細
| RRC ID | 38613 |
|---|---|
| 著者 | Ito Y, Kimura T, Ago Y, Nam K, Hiraku K, Miyazaki K, Masuzawa T, Kishida A. |
| タイトル | Nano-vibration effect on cell adhesion and its shape. |
| ジャーナル | Biomed Mater Eng |
| Abstract |
Extracellular physical features of underlying the adhesive substrate affect cell adhesion to it substrate. In this study, the effects of vibration, a type of artificial physical stimulation, on the adhesion of mouse L929 cells, mouse embryonic fibroblasts (MEFs), HeLa cells and human umbilical vein endothelial cells (HUVECs) are reported. A nano-vibration system was designed to produce nanometer-scale vibration. When L929 cells, HeLa cells, and HUVECs were subjected to vibration at 100 Hz and 1 kHz, they were not affected. On the other hand, in MEFs, the adherent cells were increased and changed their shape remarkably in response to 1 kHz vibration. To investigate the MEFs' sensitivity to vibration in detail, the cells shape was classified into four types; round, stellate, filopodia-formed and lamellipodia-formed. In serum addition culture, 1 kHz vibration increased the number of filopodia-formed cells but decreased lamellipodia cells. Furthermore, the preliminary cDNA microarray experiments showed that expression of genes which regulate cytoskeleton were changed by vibration stimulation. These results suggest that vibration could affect cell adhesion and the determination cell shape. |
| 巻・号 | 21(3) |
| ページ | 149-58 |
| 公開日 | 2011-1-1 |
| DOI | 10.3233/BME-2011-0664 |
| PII | T87G476358N0HJ06 |
| PMID | 22072079 |
| MeSH | Animals Cell Adhesion* Cell Proliferation Cell Shape* Cells, Cultured / cytology Cytoskeleton / metabolism HeLa Cells / cytology Human Umbilical Vein Endothelial Cells / cytology Humans Mice Pseudopodia / metabolism Surface Properties Vibration* |
| IF | 1.243 |
| 引用数 | 19 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929 HeLa |