論文 - 詳細
| RRC ID | 38614 |
|---|---|
| 著者 | Akihisa T, Kikuchi T, Nagai H, Ishii K, Tabata K, Suzuki T. |
| タイトル | 4-Hydroxyderricin from Angelica keiskei roots induces caspase-dependent apoptotic cell death in HL60 human leukemia cells. |
| ジャーナル | J Oleo Sci |
| Abstract |
The ethyl acetate (EtOAc)-soluble fraction of a methanol extract of Angelica keiskei roots exhibited cytotoxic activity against 4 human tumor cell lines, HL60 (leukemia), CRL1579 (melanoma), A549 (lung), and AZ521 (stomach). Nine chalcones (1-9), 5 coumarins (10-14), and 4 flavanones (15-18), isolated from the EtOAc-soluble fraction, were examined for their cytotoxic activities in the 4 human tumor cell lines. Among the compounds tested, 4-hydroxyderricin (2), a major chalcone constituent, exhibited potent cytotoxic activities in all 4 tumor cell lines with IC(50) values of 5.5 µM (HL60), 4.8 µM (CRL1579), 10.2 µM (A549), and 4.2 µM (AZ521). 4-Hydroxyderricin induced early apoptosis in HL60 cells, observed as membrane phospholipid exposure in flow cytometry. Western blot analysis showed that 4-hydroxyderricin markedly reduced the levels of procaspases-3, -8, and -9, while increasing the levels of cleaved caspases-3, -8, and -9. In addition, 4-hydroxyderricin exhibited potent inhibitory activity on human DNA topoisomerase (Topo) II (IC(50) 21.9 µM). These results suggested that 4-hydroxyderricin induces apoptotic cell death in HL60 via both the death receptor-mediated pathway and the mitochondrial pathway by, at least in part, Topo II inhibition. 4-Hydroxyderricin may therefore hold promise as an effective antitumor agent. |
| 巻・号 | 60(2) |
| ページ | 71-7 |
| 公開日 | 2011-1-1 |
| DOI | 10.5650/jos.60.71 |
| PII | JST.JSTAGE/jos/60.71 |
| PMID | 21263202 |
| MeSH | Angelica / chemistry* Annexin A5 / metabolism Apoptosis / drug effects* Blotting, Western Caspases / metabolism* Chalcone / analogs & derivatives* Chalcone / chemistry Chalcone / isolation & purification Chalcone / pharmacology DNA Topoisomerases, Type II / metabolism Drug Screening Assays, Antitumor HL-60 Cells Humans Phytotherapy Plant Roots / chemistry* Propidium / metabolism Topoisomerase Inhibitors / pharmacology |
| IF | 1.304 |
| 引用数 | 26 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) HL60(RCB0041) |