論文 - 詳細
| RRC ID | 38615 |
|---|---|
| 著者 | Akihisa T, Takahashi A, Kikuchi T, Takagi M, Watanabe K, Fukatsu M, Fujita Y, Banno N, Tokuda H, Yasukawa K. |
| タイトル | The melanogenesis-inhibitory, anti-inflammatory, and chemopreventive effects of limonoids in n-hexane extract of Azadirachta indica A. Juss. (neem) seeds. |
| ジャーナル | J Oleo Sci |
| Abstract |
Seventeen limonoids (tetranortriterpenoids 1-17) were isolated from the n-hexane extract of Azadirachta indica (neem) seeds. The previously unidentified compound 16 was established by spectroscopy to be 17-defurano-17-oxosalannin. The effects of six compounds, 6 and 11-15, on melanogenesis in B16 melanoma cells was evaluated; 2 compounds, salannin (13) and 3-deacetylsalannin (15), exhibited marked inhibitory effects (70-74% reduction of melanin content at 25 µg/mL) with only minor cytotoxicity (79-85% of cell viability). Eleven compounds, 2, 3, 5, 6, and 9-15, were evaluated for inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1.7 nmol/ear) in mice; all exhibited marked anti-inflammatory activity (ID(50) values 0.22-0.57 µmol/ear). In addition, compounds 6 and 11-16 exerted moderate inhibition (IC(50) values of 410-471 mol ratio/32 pmol TPA) of TPA-induced Epstein-Barr virus early antigen (EBV-EA) activation in Raji cells. The triacylglycerol fraction of the n-hexane extract contained oleic acid (50.2%) as the most predominant fatty acid constituent. |
| 巻・号 | 60(2) |
| ページ | 53-9 |
| 公開日 | 2011-1-1 |
| DOI | 10.5650/jos.60.53 |
| PII | JST.JSTAGE/jos/60.53 |
| PMID | 21263200 |
| MeSH | Animals Anti-Inflammatory Agents / pharmacology* Antigens, Viral / metabolism Azadirachta / chemistry* Cell Death / drug effects Chemoprevention* Fatty Acids / analysis Hexanes / chemistry* Limonins / chemistry Limonins / isolation & purification Limonins / pharmacology* Magnetic Resonance Spectroscopy Melanins / biosynthesis* Melanoma, Experimental Mice Plant Extracts / chemistry Seeds / chemistry* Tetradecanoylphorbol Acetate |
| IF | 1.304 |
| 引用数 | 30 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 murine melanoma |