| RRC ID |
38615
|
| 著者 |
Akihisa T, Takahashi A, Kikuchi T, Takagi M, Watanabe K, Fukatsu M, Fujita Y, Banno N, Tokuda H, Yasukawa K.
|
| タイトル |
The melanogenesis-inhibitory, anti-inflammatory, and chemopreventive effects of limonoids in n-hexane extract of Azadirachta indica A. Juss. (neem) seeds.
|
| ジャーナル |
J Oleo Sci
|
| Abstract |
Seventeen limonoids (tetranortriterpenoids 1-17) were isolated from the n-hexane extract of Azadirachta indica (neem) seeds. The previously unidentified compound 16 was established by spectroscopy to be 17-defurano-17-oxosalannin. The effects of six compounds, 6 and 11-15, on melanogenesis in B16 melanoma cells was evaluated; 2 compounds, salannin (13) and 3-deacetylsalannin (15), exhibited marked inhibitory effects (70-74% reduction of melanin content at 25 µg/mL) with only minor cytotoxicity (79-85% of cell viability). Eleven compounds, 2, 3, 5, 6, and 9-15, were evaluated for inhibitory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1.7 nmol/ear) in mice; all exhibited marked anti-inflammatory activity (ID(50) values 0.22-0.57 µmol/ear). In addition, compounds 6 and 11-16 exerted moderate inhibition (IC(50) values of 410-471 mol ratio/32 pmol TPA) of TPA-induced Epstein-Barr virus early antigen (EBV-EA) activation in Raji cells. The triacylglycerol fraction of the n-hexane extract contained oleic acid (50.2%) as the most predominant fatty acid constituent.
|
| 巻・号 |
60(2)
|
| ページ |
53-9
|
| 公開日 |
2011-1-1
|
| DOI |
10.5650/jos.60.53
|
| PII |
JST.JSTAGE/jos/60.53
|
| PMID |
21263200
|
| MeSH |
Animals
Anti-Inflammatory Agents / pharmacology*
Antigens, Viral / metabolism
Azadirachta / chemistry*
Cell Death / drug effects
Chemoprevention*
Fatty Acids / analysis
Hexanes / chemistry*
Limonins / chemistry
Limonins / isolation & purification
Limonins / pharmacology*
Magnetic Resonance Spectroscopy
Melanins / biosynthesis*
Melanoma, Experimental
Mice
Plant Extracts / chemistry
Seeds / chemistry*
Tetradecanoylphorbol Acetate
|
| IF |
1.304
|
| 引用数 |
30
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
|
| リソース情報 |
| ヒト・動物細胞 |
B16 murine melanoma |
