| RRC ID |
3861
|
| 著者 |
Kim YH, Takahashi M, Suzuki E, Niki E.
|
| タイトル |
Apoptosis induced by hydrogen peroxide under serum deprivation and its inhibition by antisense c-jun in F-MEL cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Under serum deprivation F-MEL cells die by apoptosis. We previously showed that apoptosis induced by serum deprivation was suppressed by inhibition of c-jun expression using antisense c-jun transfected cell line, c-junAS. To elucidate the underlying mechanisms we examined the species which is responsible for apoptosis under serum deprivation. When catalase and N-acetyl-L-cysteine (NAC) were included in the medium, cell death under serum deprivation was effectively suppressed in F-MEL cells. Intracellular generation of hydrogen peroxide (H(2)O(2)) was also detected under serum deprivation in parental F-MEL cells, but it was suppressed in c-junAS (+) cells, in which antisense c-jun was expressed and c-Jun protein expression was inhibited as shown by Western blot. When H(2)O(2) was directly applied to F-MEL cells at 3 mM, apoptotic cell death was induced, whereas it was suppressed in c-junAS (+) cells. Induction of apoptosis by H(2)O(2) and its inhibition by antisense c-jun was confirmed by detection of internucleosomal fragmentation of DNA, TdT-mediated dUTP nick end labeling (TUNEL)-positive cells and morphological alteration of nuclei. These results indicate that apoptosis induced by serum deprivation in F-MEL cells is mediated by H(2)O(2) and c-jun expression is essential to apoptosis induced by H(2)O(2) in F-MEL cells.
|
| 巻・号 |
271(3)
|
| ページ |
747-52
|
| 公開日 |
2000-5-19
|
| DOI |
10.1006/bbrc.2000.2676
|
| PII |
S0006-291X(00)92676-7
|
| PMID |
10814534
|
| MeSH |
Acetylcysteine / pharmacology
Animals
Apoptosis / drug effects*
Catalase / pharmacology
Culture Media, Serum-Free
DNA Fragmentation / drug effects
DNA, Antisense / pharmacology*
Flow Cytometry
Friend murine leukemia virus*
Gene Expression Regulation, Neoplastic / drug effects
Genes, jun / genetics*
Hydrogen Peroxide / pharmacology*
In Situ Nick-End Labeling
Leukemia, Erythroblastic, Acute*
Mice
Proto-Oncogene Proteins c-jun / metabolism
Tumor Cells, Cultured
|
| 引用数 |
41
|
|
WOS 分野
|
CHEMISTRY, MEDICINAL
PHARMACOLOGY & PHARMACY
PLANT SCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
Friend murine erythroleukemia (F-MEL) |
