RRC ID |
38630
|
著者 |
Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney JM, Rosen V, Wang EA, Tanaka H, Omura S, Suda T.
|
タイトル |
The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
The possibility that the non-osteogenic mouse pluripotent cell line, C3H10T1/2 (10T1/2), could be induced to differentiate into osteogenic cells by various hormones and cytokines was examined in vitro. Of a number of agents tested, recombinant human bone morphogenetic protein-2 (rhBMP-2) and retinoic acid induced alkaline phosphatase (ALP) activity in 10T1/2 cells. rhBMP-2 also induced mRNA expression of ALP in the cells. Dexamethasone, 1 alpha, 25-dihydroxyvitamin D3, transforming growth factor-beta 1 and insulin-like growth factor-I did not stimulate ALP activity. Treatment with rhBMP-2 greatly induced cAMP production in response to parathyroid hormone in 10T1/2 cells. No ALP activity was induced in NIH3T3 fibroblasts treated with rhBMP-2 or retinoic acid. These results indicate that 10T1/2 cells have a potential to differentiate into osteogenic cells under the control of BMP-2.
|
巻・号 |
172(1)
|
ページ |
295-9
|
公開日 |
1990-10-15
|
DOI |
10.1016/s0006-291x(05)80208-6
|
PII |
S0006-291X(05)80208-6
|
PMID |
1699539
|
MeSH |
1-Methyl-3-isobutylxanthine / pharmacology
Alkaline Phosphatase / metabolism
Animals
Blotting, Northern
Bone Morphogenetic Proteins
Cell Differentiation / drug effects*
Cell Line
Cyclic AMP / metabolism
Growth Substances / pharmacology
Humans
Kinetics
Mice
Osteoblasts / cytology*
Osteoblasts / drug effects
Osteoblasts / enzymology
Parathyroid Hormone / pharmacology
Peptide Fragments / pharmacology
Proteins / pharmacology*
RNA / isolation & purification
Recombinant Proteins / pharmacology
Teriparatide
|
IF |
2.985
|
引用数 |
422
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
10T1/2(RCB0247) |