論文 - 詳細
| RRC ID | 38630 |
|---|---|
| 著者 | Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney JM, Rosen V, Wang EA, Tanaka H, Omura S, Suda T. |
| タイトル | The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The possibility that the non-osteogenic mouse pluripotent cell line, C3H10T1/2 (10T1/2), could be induced to differentiate into osteogenic cells by various hormones and cytokines was examined in vitro. Of a number of agents tested, recombinant human bone morphogenetic protein-2 (rhBMP-2) and retinoic acid induced alkaline phosphatase (ALP) activity in 10T1/2 cells. rhBMP-2 also induced mRNA expression of ALP in the cells. Dexamethasone, 1 alpha, 25-dihydroxyvitamin D3, transforming growth factor-beta 1 and insulin-like growth factor-I did not stimulate ALP activity. Treatment with rhBMP-2 greatly induced cAMP production in response to parathyroid hormone in 10T1/2 cells. No ALP activity was induced in NIH3T3 fibroblasts treated with rhBMP-2 or retinoic acid. These results indicate that 10T1/2 cells have a potential to differentiate into osteogenic cells under the control of BMP-2. |
| 巻・号 | 172(1) |
| ページ | 295-9 |
| 公開日 | 1990-10-15 |
| DOI | 10.1016/s0006-291x(05)80208-6 |
| PII | S0006-291X(05)80208-6 |
| PMID | 1699539 |
| MeSH | 1-Methyl-3-isobutylxanthine / pharmacology Alkaline Phosphatase / metabolism Animals Blotting, Northern Bone Morphogenetic Proteins Cell Differentiation / drug effects* Cell Line Cyclic AMP / metabolism Growth Substances / pharmacology Humans Kinetics Mice Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / enzymology Parathyroid Hormone / pharmacology Peptide Fragments / pharmacology Proteins / pharmacology* RNA / isolation & purification Recombinant Proteins / pharmacology Teriparatide |
| IF | 2.985 |
| 引用数 | 422 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 104 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |