RRC ID 38630
著者 Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney JM, Rosen V, Wang EA, Tanaka H, Omura S, Suda T.
タイトル The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2.
ジャーナル Biochem Biophys Res Commun
Abstract The possibility that the non-osteogenic mouse pluripotent cell line, C3H10T1/2 (10T1/2), could be induced to differentiate into osteogenic cells by various hormones and cytokines was examined in vitro. Of a number of agents tested, recombinant human bone morphogenetic protein-2 (rhBMP-2) and retinoic acid induced alkaline phosphatase (ALP) activity in 10T1/2 cells. rhBMP-2 also induced mRNA expression of ALP in the cells. Dexamethasone, 1 alpha, 25-dihydroxyvitamin D3, transforming growth factor-beta 1 and insulin-like growth factor-I did not stimulate ALP activity. Treatment with rhBMP-2 greatly induced cAMP production in response to parathyroid hormone in 10T1/2 cells. No ALP activity was induced in NIH3T3 fibroblasts treated with rhBMP-2 or retinoic acid. These results indicate that 10T1/2 cells have a potential to differentiate into osteogenic cells under the control of BMP-2.
巻・号 172(1)
ページ 295-9
公開日 1990-10-15
DOI 10.1016/s0006-291x(05)80208-6
PII S0006-291X(05)80208-6
PMID 1699539
MeSH 1-Methyl-3-isobutylxanthine / pharmacology Alkaline Phosphatase / metabolism Animals Blotting, Northern Bone Morphogenetic Proteins Cell Differentiation / drug effects* Cell Line Cyclic AMP / metabolism Growth Substances / pharmacology Humans Kinetics Mice Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / enzymology Parathyroid Hormone / pharmacology Peptide Fragments / pharmacology Proteins / pharmacology* RNA / isolation & purification Recombinant Proteins / pharmacology Teriparatide
IF 2.985
引用数 422
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 10T1/2(RCB0247)