| RRC ID |
38632
|
| Author |
Takai T, Ohmori H.
|
| Title |
DNA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure.
|
| Journal |
Biochim Biophys Acta
|
| Abstract |
Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3.10(-5) for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively.
|
| Volume |
1048(1)
|
| Pages |
105-9
|
| Published |
1990-1-30
|
| DOI |
10.1016/0167-4781(90)90029-2
|
| PII |
0167-4781(90)90029-2
|
| PMID |
2297528
|
| MeSH |
Animals
Cell Line
DEAE-Dextran
DNA / genetics*
Electrophoresis, Agar Gel
Gene Expression
Mice
Osmotic Pressure
Transfection*
|
| IF |
3.411
|
| Times Cited |
69
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
P3/NSI/1-AG4-1(RCB0095) |
