論文 - 詳細
| RRC ID | 38645 |
|---|---|
| 著者 | Hirano Y, Kando Y, Hayashi T, Goto K, Nakajima A. |
| タイトル | Synthesis and cell attachment activity of bioactive oligopeptides: RGD, RGDS, RGDV, and RGDT. |
| ジャーナル | J Biomed Mater Res |
| Abstract |
Tetrapeptides, Arg-Gly-Asp-Ser (RGDS), Arg-Gly-Asp-Val (RGDV), and Arg-Gly-Asp-Thr (RGDT), present in the cell-attachment domain of fibronectin, vitronectin, and collagen, respectively, were synthesized by using an improved liquid-phase procedure. Bioactivities of RGD and RGDX (X = S, V, and T) as cell recognition determinants were investigated by two methods to evaluate interactions of these oligopeptides with L-929 fibroblast cells originating in mouse epithelia. In the first method, these oligopeptides were immobilized to ethylene-acrylic acid copolymer (EAA) film, and the cell-attachment activity of the immobilized film was measured. In the second method, interaction of oligopeptides with the cells was evaluated by measuring the cell inhibition caused by oligopeptides. It was found that RGD and RGDX exhibit remarkable cell-attachment activity, and the activity of oligopeptides depends on X. |
| 巻・号 | 25(12) |
| ページ | 1523-34 |
| 公開日 | 1991-12-1 |
| DOI | 10.1002/jbm.820251209 |
| PMID | 1724445 |
| MeSH | Amino Acid Sequence Animals Cell Adhesion Collagen / chemistry Epithelial Cells Fibroblasts / drug effects Fibroblasts / metabolism Fibronectins / chemistry Glycoproteins / chemistry Mice Molecular Sequence Data Oligopeptides / chemical synthesis Oligopeptides / pharmacology* Temperature Vitronectin |
| 引用数 | 38 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB0081) |