| RRC ID |
38652
|
| 著者 |
Tanida N, Ohno N, Adachi Y, Matsuura M, Nakano M, Kiso M, Hasegawa A, Yadomae T.
|
| タイトル |
Modification of immunopharmacological activities of synthetic monosaccharide lipid A analogue, GLA60, by lysozyme.
|
| ジャーナル |
J Biochem
|
| Abstract |
Recent studies by our group suggested that lysozyme has a high affinity for bacterial lipopolysaccharide (LPS) of both the smooth and rough forms, and inhibits various immunomodulatory activities of LPS. GLA60 is a synthetic monosaccharide analogue of bacterial lipid A well known as having most of the activities of lipid A with very low toxicity. In this study, we characterized the interaction of lysozyme with GLA60 in comparison to that with Escherichia coli 0111 LPS (smooth form) by means of an immunopharmacological approach. Using dansylated lysozyme (DNS-LZM) as a probe, LZM was found to bind to GLA60. The mitogenic and polyclonal B-cell activating activities were significantly reduced by complex formation. However, there was no inhibitory effect on GLA60 induced production of IL-1 and TNF of macrophages. Interestingly, the activities of macrophages induced by the complex were found to be significantly higher than those induced by GLA60 itself. In contrast, the activities of 0111 LPS were significantly inhibited by LZM. Since the GLA60-LZM complex produced a turbid suspension but the 0111 LPS-LZM complex remained soluble, we consider that the activities of GLA60 alone were mediated by the common functional LPS receptor for dispersed form in both macrophages and B-lymphocytes, but activation of macrophages by the complex was mediated either by another LPS receptor not present in B-lymphocytes or through the phagocytic function of macrophages.
|
| 巻・号 |
112(5)
|
| ページ |
616-23
|
| 公開日 |
1992-11-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a123949
|
| PMID |
1478920
|
| MeSH |
Adjuvants, Immunologic / metabolism
Adjuvants, Immunologic / pharmacology*
Animals
B-Lymphocytes / drug effects
B-Lymphocytes / immunology
Cell Line
Dansyl Compounds / chemistry
Interleukin-1 / biosynthesis
Lipid A / analogs & derivatives*
Lipid A / metabolism
Lipid A / pharmacology
Macrophages / drug effects
Macrophages / metabolism
Male
Mice
Mice, Inbred C3H
Mitogens
Muramidase / metabolism*
Tumor Necrosis Factor-alpha / biosynthesis
|
| IF |
2.476
|
| 引用数 |
15
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
