RRC ID |
38658
|
著者 |
Obata K, Iwata K, Okada Y, Kohrin Y, Ohuchi E, Yoshida S, Shinmei M, Hayakawa T.
|
タイトル |
A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies.
|
ジャーナル |
Clin Chim Acta
|
Abstract |
A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 micrograms/l and linearity was obtained between 31 and 500 micrograms/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.
|
巻・号 |
211(1-2)
|
ページ |
59-72
|
公開日 |
1992-10-15
|
DOI |
10.1016/0009-8981(92)90105-y
|
PII |
0009-8981(92)90105-Y
|
PMID |
1281763
|
MeSH |
Animals
Antibodies, Monoclonal*
Antibody Specificity
Cell Line
Female
Glycoproteins / metabolism
Humans
Immunoenzyme Techniques* / standards
Immunoenzyme Techniques* / statistics & numerical data
Matrix Metalloproteinase 3
Metalloendopeptidases / analysis*
Metalloendopeptidases / blood
Metalloendopeptidases / immunology
Mice
Mice, Inbred BALB C
Neoplasm Proteins / metabolism
Protein Precursors / analysis
Protein Precursors / blood
Protein Precursors / immunology
Synovial Fluid / chemistry
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinases
alpha-Macroglobulins / metabolism
|
IF |
2.615
|
引用数 |
121
|
WOS 分野
|
MEDICAL LABORATORY TECHNOLOGY
|
リソース情報 |
ヒト・動物細胞 |
NB1RGB(RCB0222) |