RRC ID 38658
著者 Obata K, Iwata K, Okada Y, Kohrin Y, Ohuchi E, Yoshida S, Shinmei M, Hayakawa T.
タイトル A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies.
ジャーナル Clin Chim Acta
Abstract A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 micrograms/l and linearity was obtained between 31 and 500 micrograms/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.
巻・号 211(1-2)
ページ 59-72
公開日 1992-10-15
DOI 10.1016/0009-8981(92)90105-y
PII 0009-8981(92)90105-Y
PMID 1281763
MeSH Animals Antibodies, Monoclonal* Antibody Specificity Cell Line Female Glycoproteins / metabolism Humans Immunoenzyme Techniques* / standards Immunoenzyme Techniques* / statistics & numerical data Matrix Metalloproteinase 3 Metalloendopeptidases / analysis* Metalloendopeptidases / blood Metalloendopeptidases / immunology Mice Mice, Inbred BALB C Neoplasm Proteins / metabolism Protein Precursors / analysis Protein Precursors / blood Protein Precursors / immunology Synovial Fluid / chemistry Tissue Inhibitor of Metalloproteinase-2 Tissue Inhibitor of Metalloproteinases alpha-Macroglobulins / metabolism
IF 2.615
引用数 121
WOS 分野 MEDICAL LABORATORY TECHNOLOGY
リソース情報
ヒト・動物細胞 NB1RGB(RCB0222)