Reference - Detail
| RRC ID | 38661 |
|---|---|
| Author | Takai T, Ohmori H. |
| Title | Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells. |
| Journal | Biochim Biophys Acta |
| Abstract |
The expression of genes introduced into various mammalian cell lines was enhanced by raising the temperature of the cells to 42 degrees C for a few hours after DNA transfection. This heat treatment resulted in an up to 10-fold increase in the frequency of the cells that transiently expressed a foreign gene such as that of beta-galactosidase, whereas it had only a limited enhancing effect on the development of stable transformants. By immunotitration analysis, it was confirmed that the enhanced expression of beta-galactosidase activity correlated well with the increase of the enzyme protein. This procedure may have an applicability for augmenting the frequency of transient gene expression in many cell types. |
| Volume | 1129(2) |
| Pages | 161-5 |
| Published | 1992-1-6 |
| DOI | 10.1016/0167-4781(92)90481-e |
| PII | 0167-4781(92)90481-E |
| PMID | 1730054 |
| MeSH | Animals Cell Line Escherichia coli / enzymology Escherichia coli / genetics Gene Expression Genetic Vectors Hot Temperature* Immunochemistry / methods Plasmids Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Transfection* beta-Galactosidase / biosynthesis beta-Galactosidase / genetics |
| IF | 3.411 |
| Times Cited | 14 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | P3/NSI/1-AG4-1(RCB0095) |