| RRC ID |
38671
|
| Author |
Kajimoto Y, Kawamori R, Umayahara Y, Iwama N, Imano E, Morishima T, Yamasaki Y, Kamada T.
|
| Title |
An AP-1 enhancer mediates TPA-induced transcriptional activation of the chicken insulin-like growth factor I gene.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
As a step to elucidate a role of protein kinase C(PKC) pathways in the regulation of insulin-like growth factor I(IGF-I) gene, we sought to determine whether the IGF-I gene promoter of chicken can be a target of regulation by PKC. An initial gene transfer study showed that, in a human cell line HepG2, the IGF-I gene promoter directs accurate transcription of IGF-I-luciferase fusion gene and enhances luciferase activity. Treatment of transfected cells with 12-o-tetradecanoylphorbol 13-acetate(TPA) increased promoter activity of 2100 and 600bp 5'-flanking sequence 4.9- and 3.6-fold, respectively. Site-directed mutagenesis in the AP-1-like sequence located within the 600bp resulted in 91% loss of its TPA-induced promoter activity, and a gel mobility-shift analysis revealed that TPA-stimulation of HepG2 cells caused a dramatic increase in specific protein-binding to the AP-1-like sequence, suggesting that the sequence functions as an AP-1 enhancer. These observations support a direct role for PKC pathways in activating the IGF-I gene promoter in chicken.
|
| Volume |
190(3)
|
| Pages |
767-73
|
| Published |
1993-2-15
|
| DOI |
10.1006/bbrc.1993.1115
|
| PII |
S0006-291X(83)71115-0
|
| PMID |
8439327
|
| MeSH |
Animals
Base Sequence
Binding Sites
Chickens
DNA-Binding Proteins / metabolism
Enhancer Elements, Genetic*
Gene Expression Regulation*
Insulin-Like Growth Factor I / genetics*
Molecular Sequence Data
Mutagenesis, Site-Directed
Promoter Regions, Genetic*
Proto-Oncogene Proteins c-jun / metabolism
Tetradecanoylphorbol Acetate / pharmacology*
Transcription, Genetic / drug effects*
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB0459) |
