| RRC ID |
38674
|
| Author |
Fujiwara T, Motoyama T, Ishihara N, Watanabe H, Kumanishi T, Kato K, Ichinose H, Nagatsu T.
|
| Title |
Characterization of four new cell lines derived from small-cell gastrointestinal carcinoma.
|
| Journal |
Int J Cancer
|
| Abstract |
Four human small-cell gastrointestinal carcinoma cell lines were established from tumor tissues of patients with esophageal, gastric or rectal cancer, and were studied morphologically and biochemically in comparison with small-cell lung carcinoma (SCLC) cell lines and common gastric cancer cell lines. Cells from all the small-cell gastrointestinal carcinoma lines were as small as classic SCLC cells and had characteristic neurosecretory granules. Cells from only one line grew as tightly packed spherical aggregates of floating cells, and those of the other 3 grew attached to substrate. Although high levels of creatine kinase brain isoenzyme (CK-BB) were detected in all 4 cell lines, 2 of them showed low levels of aromatic L-amino-acid decarboxylase and 3 had low levels of neuron-specific enolase (NSE). None of the lines showed simultaneous elevation of enzymes. C-myc, N-myc, and L-myc were not amplified in any of the cell lines, but c-myc mRNA was expressed in 2 lines. Our findings indicate that all small-cell gastrointestinal carcinoma cells examined belong to the variant type which is used in the classification of SCLC. Furthermore, the ECC18 line, derived from esophageal cancer, seemed to be of true endocrine cell origin, while the 3 other small-cell gastrointestinal carcinoma lines seemed to arise via neoplastic neometaplasia from adenocarcinoma cells to endocrine cells.
|
| Volume |
54(6)
|
| Pages |
965-71
|
| Published |
1993-7-30
|
| DOI |
10.1002/ijc.2910540617
|
| PMID |
8392984
|
| MeSH |
Aged
Animals
Carcinoma, Small Cell / chemistry
Carcinoma, Small Cell / genetics
Carcinoma, Small Cell / pathology*
DNA, Neoplasm / analysis
Gastrointestinal Neoplasms / chemistry
Gastrointestinal Neoplasms / genetics
Gastrointestinal Neoplasms / pathology*
Gene Expression
Genes, myc
Humans
Lung Neoplasms / pathology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Transplantation
RNA, Neoplasm / analysis
Tumor Cells, Cultured
|
| IF |
5.145
|
| Times Cited |
15
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
Lu-130(RCB0465)
Lu-139(RCB0469) |
