論文 - 詳細
| RRC ID | 38676 |
|---|---|
| 著者 | Fujimoto N, Mouri N, Iwata K, Ohuchi E, Okada Y, Hayakawa T. |
| タイトル | A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 2 (72-kDa gelatinase/type IV collagenase) using monoclonal antibodies. |
| ジャーナル | Clin Chim Acta |
| Abstract |
A one-step sandwich enzyme immunoassay (EIA) for human matrix metalloproteinase 2 (MMP-2, 72-kDa gelatinase/type IV collagenase, EC 3.4.24.24) was established with a pair of monoclonal antibodies prepared against the precursor form of MMP-2 (proMMP-2) purified from the conditioned medium of human skin fibroblasts or against a synthetic peptide corresponding to the N-terminal domain of proMMP-2. ProMMP-2 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of this EIA system was 2.4 pg/assay (0.24 microgram/l) and linearity was obtained between 10 and 5,000 pg/assay (1.0-500 micrograms/l). The EIA system recognized both the free form of proMMP-2 and its complex form with TIMP-2 with the same degree of immunoreactivity. ProMMP-2 levels in human sera from patients in various disease states were analyzed. In sera from patients with hyperthyroidism (12), primary biliary cirrhosis (8) and hepatocellular carcinoma (11), 749 +/- 166, 716 +/- 135 and 686 +/- 236 micrograms/l of proMMP-2 were detected, respectively and these were significantly higher than that observed in 213 normal human sera (570 +/- 118 micrograms/l). In contrast, the levels in sera from 33 patients with osteoarthritis (449 +/- 72 micrograms/l), 45 with rheumatoid arthritis (408 +/- 139 micrograms/l), 13 with stomach cancer (427 +/- 103 micrograms/l) and 10 with pancreatic cancer (422 +/- 130 micrograms/l) were significantly lower than that found in normal sera. Immunoblot and gel filtration analyses showed that human sera contain several MMP-2 species in addition to proMMP-2 which exist in a complex form with TIMP-2. |
| 巻・号 | 221(1-2) |
| ページ | 91-103 |
| 公開日 | 1993-11-30 |
| DOI | 10.1016/0009-8981(93)90024-x |
| PII | 0009-8981(93)90024-X |
| PMID | 8149645 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal* Antibody Specificity Arthritis, Rheumatoid / enzymology Evaluation Studies as Topic Female Gelatinases / blood* Gelatinases / isolation & purification Humans Hyperthyroidism / enzymology Immunoenzyme Techniques Liver Cirrhosis, Biliary / enzymology Matrix Metalloproteinase 2 Metalloendopeptidases / blood* Metalloendopeptidases / isolation & purification Mice Mice, Inbred BALB C Molecular Sequence Data Neoplasms / enzymology Protein Precursors / blood* Protein Precursors / immunology Protein Precursors / isolation & purification Proteins / analysis Proteins / immunology Reference Standards Tissue Inhibitor of Metalloproteinase-2 |
| IF | 2.615 |
| 引用数 | 163 |
| WOS 分野 | MEDICAL LABORATORY TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |