| RRC ID |
38682
|
| 著者 |
Ohmori H, Ikeda H, Tanigawa T, Takai T, Hikida M.
|
| タイトル |
Enzyme release assay of human NK cell activity using beta-galactosidase-expressing K562 target cell line.
|
| ジャーナル |
J Immunol Methods
|
| Abstract |
In the present report, we established a K562 cell line useful for an enzyme release assay of human natural killer (NK) activity. Human myelogenous leukemia cell line, K562, was transfected with a plasmid carrying Escherichia coli beta-galactosidase (beta-gal) gene. A colony that permanently expresses the enzyme activity was isolated, and designated K562/Zneo. Incubation of K562/Zneo cells (1 x 10(4)) with nonadherent human peripheral blood lymphocytes (PBL) resulted in the release of beta-gal activity depending on the incubation time and the number of effector cells. Released beta-gal activity was assayed sensitively by using 4-methylumbelliferyl-beta-D-galactoside, a fluorescent substrate. The cytolytic activity of PBL was augmented significantly when the cells were preincubated with interleukin-2 for 20 h. This enzyme release assay showed a comparable sensitivity to that of 51Cr release assay. Thus, K562/Zneo cell line is thought to be useful for the nonradioactive assay of human NK and lymphokine-activated killer activities.
|
| 巻・号 |
164(1)
|
| ページ |
131-5
|
| 公開日 |
1993-8-26
|
| DOI |
10.1016/0022-1759(93)90283-d
|
| PII |
0022-1759(93)90283-D
|
| PMID |
8360503
|
| MeSH |
Cytotoxicity, Immunologic*
Humans
Immunity, Cellular
In Vitro Techniques
Killer Cells, Natural / immunology*
Leukemia, Myeloid
Transfection
Tumor Cells, Cultured
beta-Galactosidase / genetics*
|
| IF |
1.901
|
| 引用数 |
5
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
