RRC ID 38688
著者 Murasawa S, Matsubara H, Urakami M, Inada M.
タイトル Regulatory elements that mediate expression of the gene for the angiotensin II type 1a receptor for the rat.
ジャーナル J Biol Chem
Abstract To analyze the mechanism of the cell type-specific expression of rat angiotensin II type 1a receptor (AT1a-R) gene, we isolated the 5'-portion of the gene and identified multiple positive and negative regulatory sequences that regulate its transcription. Primer extension and S1 mapping identified a transcriptional initiation site at 33 (position +1) base pairs (bp) downstream of TATA sequence. The transcriptional activities of various 5'-deletion mutants of the AT1a-R gene upstream region, fused to the chloramphenicol acetyltransferase (CAT) gene, were examined using rat vascular smooth muscle cells (A10) and glial cells expressing AT1a-R mRNA predominantly or PC12 cells expressing AT2-R and very small amounts of AT1a-R mRNA. A 980-bp 5'-flanking sequence contained at least three positive elements, P1 (-560 to -489), P2 (-331 to -201), and P3 (-201 to -61). P1 and P3 were active in the tested three cells, and P2 was functional only in glial and PC12 cells. In addition to these positive elements, there was negative element, N1 (-489 to -331), which was active only in PC12 cells. These elements, when cotransfected with the AT1a-CAT fusion gene, had competitive effects against its promoter activity. The present study suggests the presence of multiple trans-acting factors that act on these positive and negative cis-acting elements and regulate the cell type-specific expression of the rat AT1a-R gene.
巻・号 268(36)
ページ 26996-7003
公開日 1993-12-25
PII S0021-9258(19)74209-4
PMID 8262935
MeSH Animals Base Sequence Chloramphenicol O-Acetyltransferase / genetics DNA, Complementary Gene Expression Regulation Molecular Sequence Data Neuroglia / cytology Neuroglia / metabolism PC12 Cells Rats Receptors, Angiotensin / genetics* Regulatory Sequences, Nucleic Acid* Transcription, Genetic Transfection
IF 4.238
引用数 54
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)