RRC ID 38693
Author Yoshiura M, Murakami H, Tashiro H, Kurisu K.
Title Production of a human monoclonal antibody to normal basal and squamous cell carcinoma-associated antigen.
Journal J. Oral Pathol. Med.
Abstract A human monoclonal antibody, BM2, was produced by a hybridoma line generated by fusion of lymph node cells from a patient with squamous cell carcinoma (SCC) of the tongue with human B-lymphoblastoid cell line HO-323. BM2, an IgM class antibody, was reactive with all of the SCC cell lines tested. Frozen sections of normal and malignant tumor specimens were investigated to examine the reactivity of BM2 towards them. All 35 oral SCC specimens reacted with BM2. Normal stratified squamous epithelium showed positive staining in basal cells, but no staining was seen in other layers of the stratified epithelium, simple epithelium, and tissues of nonepithelial origin. Ductal basal cells of normal salivary gland also showed positive staining. Western blotting and immunoprecipitation analysis revealed that BM2 recognized 52 kDa membrane-associated protein. BM2 may therefore be a useful tool for biological and clinical studies of SCC.
Volume 22(10)
Pages 451-8
Published 1993-11
DOI 10.1111/j.1600-0714.1993.tb00124.x
PMID 7510337
MeSH Adenocarcinoma / immunology Antibodies, Monoclonal / biosynthesis* Antibodies, Monoclonal / drug effects Antigens, Neoplasm / immunology* Carcinoma, Squamous Cell / immunology* Epithelial Cells Epithelium / immunology Fluorescent Antibody Technique Formaldehyde / pharmacology Humans Hybridomas / immunology Immunoglobulin Isotypes / biosynthesis Immunoglobulin Isotypes / drug effects Immunoglobulin M / biosynthesis Immunoglobulin M / drug effects Immunohistochemistry Keratins Membrane Proteins / immunology Mitosis / immunology Mouth Mucosa / cytology Mouth Mucosa / immunology* Neuraminidase / pharmacology Periodic Acid / pharmacology Pronase / pharmacology Submandibular Gland / cytology Submandibular Gland / immunology Tumor Cells, Cultured
IF 2.237
Times Cited 2
Human and Animal Cells