Reference - Detail
| RRC ID | 38702 |
|---|---|
| Author | Hashimoto E, Kage K, Ogita T, Nakaoka T, Matsuoka R, Kira Y. |
| Title | Adenosine as an endogenous mediator of hypoxia for induction of vascular endothelial growth factor mRNA in U-937 cells. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Adenosine induced by hypoxia exerts various effects via different types of receptors. Recently, hypoxia was shown to be a strong inducer of vascular endothelial growth factor, a secreted endothelial cell specific mitogen. In this report, we studied on effects of adenosine on inducibility of VEGF and possible mediation of hypoxia for its induction in U-937 cells. Hypoxia induced expression of VEGF mRNA with an early peak at 1 hour. 5'-N-ethylcarboxamidoadenosine, an adenosine analog, strongly induced VEGF mRNA, which was inhibited by 3,7-dimethyl-1-propargylxanthine (DMPX), an A2-antagonist. The hypoxic induction was inhibited by adenosine deaminase, 7-(beta-hydroxyethyl)theophylline, a non-selective adenosine receptor antagonist and DMPX. These results suggest that the hypoxic induction of VEGF mRNA is mediated by adenosine via A2-receptor in U-937 cells. |
| Volume | 204(1) |
| Pages | 318-24 |
| Published | 1994-10-14 |
| DOI | 10.1006/bbrc.1994.2462 |
| PII | S0006-291X(84)72462-4 |
| PMID | 7945378 |
| MeSH | Adenosine / analogs & derivatives* Adenosine / pharmacology Adenosine / physiology* Adenosine Deaminase / pharmacology Adenosine-5'-(N-ethylcarboxamide) Base Sequence Blotting, Northern Bucladesine / pharmacology Cell Hypoxia Cell Line Cloning, Molecular DNA Primers Endothelial Growth Factors / biosynthesis* Gene Expression* / drug effects Humans Lymphokines / biosynthesis* Molecular Sequence Data Polymerase Chain Reaction / methods Purinergic P1 Receptor Antagonists RNA, Messenger / analysis RNA, Messenger / biosynthesis Theobromine / analogs & derivatives Theobromine / pharmacology Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| IF | 2.985 |
| Times Cited | 57 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | U-937 DE-4(RCB0435) |