| RRC ID |
38706
|
| 著者 |
Matsumura M, Niwa Y, Kato N, Komatsu Y, Shiina S, Kawabe T, Kawase T, Toyoshima H, Ihori M, Shiratori Y.
|
| タイトル |
Detection of alpha-fetoprotein mRNA, an indicator of hematogenous spreading hepatocellular carcinoma, in the circulation: a possible predictor of metastatic hepatocellular carcinoma.
|
| ジャーナル |
Hepatology
|
| Abstract |
We attempted to detect circulating hepatocellular carcinoma by demonstrating hepatocyte-associated mRNA in the nuclear cell component of peripheral blood using nested reverse transcription-polymerase chain reaction because of the extremely small number of tumor cells in the circulation. Albumin mRNA was demonstrated not only in the liver tissue (hepatocytes) and HepG2 cells but also in nuclear cells of the blood from normal healthy volunteers (neutrophils and lymphocytes) by reverse transcription-polymerase chain reaction. In contrast, alpha-fetoprotein mRNA was demonstrated in the liver tissue, as well as in HepG2 cells, but not in peripheral blood of normal healthy volunteers, indicating the possibility of using alpha-fetoprotein mRNA for detection of benign and malignant hepatocytes among the population of neutrophils and lymphocytes. alpha-Fetoprotein mRNA in peripheral blood was detected in 17 of 33 cases of hepatocellular carcinoma (52%), 2 of 13 cases of cirrhosis (15%) and 2 of 17 cases of chronic hepatitis (12%). alpha-Fetoprotein mRNA was not demonstrated in 26 cases of normal healthy volunteers (0%). Among the patients with hepatocellular carcinoma, total volume of tumor tissue, maximum size of tumor and serum alpha-fetoprotein level were markedly increased in the patients with alpha-fetoprotein mRNA in blood. In addition, alpha-fetoprotein mRNA was detected in the blood of all 6 patients showing metastasis at extrahepatic organs (100%), in contrast to 11 of 27 cases without metastasis (41%). From these results, we conclude that the presence of alpha-fetoprotein mRNA in peripheral blood may be an indicator of circulating malignant or benign hepatocytes, which might predict hematogenous spreading metastasis of tumor cells in patients with hepatocellular carcinoma.
|
| 巻・号 |
20(6)
|
| ページ |
1418-25
|
| 公開日 |
1994-12-1
|
| DOI |
10.1002/hep.1840200607
|
| PII |
S0270913994003629
|
| PMID |
7527002
|
| MeSH |
Adult
Aged
Aged, 80 and over
Albumins / genetics
Base Sequence
Carcinoma, Hepatocellular / blood*
Carcinoma, Hepatocellular / chemistry
Carcinoma, Hepatocellular / pathology
Chi-Square Distribution
Female
Humans
Liver / chemistry
Liver Neoplasms / blood*
Liver Neoplasms / chemistry
Liver Neoplasms / pathology
Male
Middle Aged
Molecular Sequence Data
Neoplasm Metastasis
Neoplastic Cells, Circulating / metabolism*
Neoplastic Cells, Circulating / pathology
Polymerase Chain Reaction / methods
Predictive Value of Tests
RNA, Messenger / analysis
RNA, Messenger / blood*
Transcription, Genetic
alpha-Fetoproteins / genetics*
|
| IF |
14.679
|
| 引用数 |
129
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2(RCB459) |
