論文 - 詳細
| RRC ID | 38711 |
|---|---|
| 著者 | Imamura H, Takao S, Aikou T. |
| タイトル | A modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay for quantitating tumor cell invasion. |
| ジャーナル | Cancer Res |
| Abstract |
Reconstituted basement membrane matrix (Matrigel) has been utilized for in vitro assay of tumor cell invasion in recent years. In the conventional chamber for the invasion assay, however, a large number of cells passed easily through the center of the Matrigel-coated filter because the Matrigel layer could not be completely uniform by the meniscus formation. To prevent the meniscus phenomenon of the Matrigel layer, we devised a water-repellent treatment of the inside wall of the assay chamber with paraffin. Consequently, very few erythrocytes passed through the Matrigel-coated filter of this modified chamber with the erythrocyte assay, which was used to demonstrate the evenness and uniformity of the Matrigel layer on the filter. For quantitating a small number of cells which invaded through the Matrigel-coated filter by the invasion assay, a tetrazolium-based colorimetric 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay was used. The invasive abilities of the eight different cells were determined by this invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium+ ++ bromide assay using the modified chamber with a filter coated with 70 microliters of the 0.2-mg/ml Matrigel. After 72 h of incubation, the malignant cell lines significantly exceeded the normal cell lines in the percentage of invasion (P < 0.01). Therefore, the modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium+ ++ bromide assay provides a simple, easily reproducible in vitro assay for quantitating tumor cell invasion. |
| 巻・号 | 54(13) |
| ページ | 3620-4 |
| 公開日 | 1994-7-1 |
| PMID | 8012990 |
| MeSH | Animals Collagen* Colorimetry Coloring Agents* Diffusion Chambers, Culture / methods* Drug Combinations Erythrocytes / pathology Humans Laminin* Mice Neoplasm Invasiveness / pathology Neoplasm Invasiveness / physiopathology* Proteoglycans* Tetrazolium Salts* Thiazoles* Vero Cells / pathology |
| IF | 9.727 |
| 引用数 | 49 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) NIH3T3/14-1(RCB0056) MRC-5 SV1 TG1(RCB0207) |